Document Detail


Isolation and fractionation of ferritin from human term placenta--a source for human isoferritins.
MedLine Citation:
PMID:  3922239     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for isolating ferritin from human term placenta was described. The placenta was homogenized in water containing protease inhibitor and heated at 70 degrees C. The ferritin was precipitated with ammonium sulfate at pH 5.2 and purified by repeated cycles of ultracentrifugation and molecular sieve chromatography through Sepharose 4B columns. Isoelectric focusing revealed a broad spectrum of isoferritins. These isoferritins were separated by ion-exchange chromatography on Sephadex A-25 at pH 7.5 and stepwise elution with increasing concentrations of NaCl. By this method "basic," "intermediate," and "acid" isoferritins were separated. The most basic placental isoferritin was shown to be identical to splenic ferritin by isoelectric focusing, subunit analysis, and fluorescent ELISA. The acid placental isoferritin had similar characteristics to heart-type ferritin. It was suggested that the easily available placental tissue could serve as a source for human isoferritins in research and in clinical assays.
Authors:
A M Konijn; R Tal; R Levy; Y Matzner
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Analytical biochemistry     Volume:  144     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1985 Feb 
Date Detail:
Created Date:  1985-06-19     Completed Date:  1985-06-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  423-8     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Carbonic Anhydrases / blood
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Female
Ferritins / analysis*,  isolation & purification
Humans
Isoelectric Focusing
Molecular Weight
Placenta / analysis*
Pregnancy
Spleen / metabolism
Thalassemia / metabolism
Ultracentrifugation
Chemical
Reg. No./Substance:
9007-73-2/Ferritins; EC 4.2.1.1/Carbonic Anhydrases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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