Document Detail

Isolation of food allergens.
MedLine Citation:
PMID:  11419729     Owner:  NLM     Status:  MEDLINE    
The identification of food allergens is a priority in the management of food allergy, because of the need to obtain standardized extracts and pure allergens for diagnosis and therapy. It is thus important to develop methods for purification of allergenic molecules in order to study their biological and immunological characteristics. Protocols for protein extraction from foods and for allergen purification are reviewed in this paper. We report published methods for extraction of allergens from either animal and vegetable foods and detailed purification methodologies including ion-exchange, gel filtration and reversed-phase chromatography of well known allergens.
E A Pastorello; C Trambaioli
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Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  756     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-22     Completed Date:  2001-12-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-84     Citation Subset:  IM    
3rd Division of General Medicine, Ospedale Maggiore IRCCS, Milan, Italy.
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MeSH Terms
Allergens / isolation & purification*
Chromatography, Liquid / methods*
Electrophoresis, Polyacrylamide Gel / methods
Reg. No./Substance:

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