| Isolation of food allergens. | |
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MedLine Citation:
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PMID: 11419729 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The identification of food allergens is a priority in the management of food allergy, because of the need to obtain standardized extracts and pure allergens for diagnosis and therapy. It is thus important to develop methods for purification of allergenic molecules in order to study their biological and immunological characteristics. Protocols for protein extraction from foods and for allergen purification are reviewed in this paper. We report published methods for extraction of allergens from either animal and vegetable foods and detailed purification methodologies including ion-exchange, gel filtration and reversed-phase chromatography of well known allergens. |
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Authors:
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E A Pastorello; C Trambaioli |
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Publication Detail:
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Type: Journal Article; Review |
Journal Detail:
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Title: Journal of chromatography. B, Biomedical sciences and applications Volume: 756 ISSN: 1387-2273 ISO Abbreviation: J. Chromatogr. B Biomed. Sci. Appl. Publication Date: 2001 May |
Date Detail:
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Created Date: 2001-06-22 Completed Date: 2001-12-05 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 9714109 Medline TA: J Chromatogr B Biomed Sci Appl Country: Netherlands |
Other Details:
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Languages: eng Pagination: 71-84 Citation Subset: IM |
Affiliation:
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3rd Division of General Medicine, Ospedale Maggiore IRCCS, Milan, Italy. elidepa@box1.tin.it |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergens
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isolation & purification* Animals Chromatography, Liquid / methods* Electrophoresis, Polyacrylamide Gel / methods Food* Mice |
| Chemical | |
Reg. No./Substance:
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0/Allergens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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