Document Detail


Isolation of food allergens.
MedLine Citation:
PMID:  11419729     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The identification of food allergens is a priority in the management of food allergy, because of the need to obtain standardized extracts and pure allergens for diagnosis and therapy. It is thus important to develop methods for purification of allergenic molecules in order to study their biological and immunological characteristics. Protocols for protein extraction from foods and for allergen purification are reviewed in this paper. We report published methods for extraction of allergens from either animal and vegetable foods and detailed purification methodologies including ion-exchange, gel filtration and reversed-phase chromatography of well known allergens.
Authors:
E A Pastorello; C Trambaioli
Related Documents :
2150429 - Allergic manifestations due to castor beans: an undue risk for the dock workers handlin...
7278739 - Placebo-controlled tests of chemical food additives: are they valid?
12487199 - Stability of bovine allergens during food processing.
3409789 - Reducing house-dust mites by vacuuming.
19671379 - Prevention of allergic sensitization by environmental control.
17245089 - Hypoallergenicity: a principle for the treatment of food allergy.
11082059 - Regional changes in carbon dioxide fluxes of land and oceans since 1980.
16957679 - Consensus meeting: monosodium glutamate - an update.
17617459 - Heavy metal (cd, pb, zn) and metalloid (as) content in raptor species from galicia (nw ...
Publication Detail:
Type:  Journal Article; Review    
Journal Detail:
Title:  Journal of chromatography. B, Biomedical sciences and applications     Volume:  756     ISSN:  1387-2273     ISO Abbreviation:  J. Chromatogr. B Biomed. Sci. Appl.     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-06-22     Completed Date:  2001-12-05     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  9714109     Medline TA:  J Chromatogr B Biomed Sci Appl     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  71-84     Citation Subset:  IM    
Affiliation:
3rd Division of General Medicine, Ospedale Maggiore IRCCS, Milan, Italy. elidepa@box1.tin.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Allergens / isolation & purification*
Animals
Chromatography, Liquid / methods*
Electrophoresis, Polyacrylamide Gel / methods
Food*
Mice
Chemical
Reg. No./Substance:
0/Allergens

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Standardization of food allergen extracts for skin prick test.
Next Document:  The major allergen of sesame seeds (Sesamum indicum) is a 2S albumin.