Document Detail


Isolation and fluorescence spectrum of cardiac myosin from pig heart.
MedLine Citation:
PMID:  19475499     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cardiac myosin is the most abundant and bioactive protein presented in heart tissue. In this work, a simple method was described for preparation of high purity cardiac myosin for further research. With the use of a domestic pig left ventricle as the starting tissue, this method yielded 4.5 mg myosin per gram of wet tissue with a specific activity of 0.293 U/mg. The purification factor was 4.9-fold with an activity recovery of 42.1%. In addition, the investigation of fluorescence spectrum shown that the emission intensity of cardiac myosin has a dramatic decrease under the influence of an acidic environment or at temperatures higher than 30 degrees C, which could influence the normal physiological function of cardiac myosin.
Authors:
Yang Liu; Buhai Li; Xiaomei Sun; Aihua Lin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The protein journal     Volume:  28     ISSN:  1875-8355     ISO Abbreviation:  Protein J.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-07-13     Completed Date:  2009-09-28     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101212092     Medline TA:  Protein J     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  169-74     Citation Subset:  IM    
Affiliation:
School of Life Science and Technology, Wuhan University of Science and Technology Zhongnan Branch, 430223 Wuhan, Hubei, People's Republic of China. znmdly@sina.com
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MeSH Terms
Descriptor/Qualifier:
Ammonium Sulfate / chemistry
Animals
Cardiac Myosins / chemistry*,  isolation & purification
Chromatography, High Pressure Liquid
Electrophoresis, Polyacrylamide Gel
Heart Ventricles / chemistry
Hydrogen-Ion Concentration
Myocardium / chemistry*
Spectrometry, Fluorescence / methods*
Swine
Temperature
X-Ray Diffraction
Chemical
Reg. No./Substance:
7783-20-2/Ammonium Sulfate; EC 3.6.1.-/Cardiac Myosins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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