| Isolation of fetal trophoblasts and nucleated erythrocytes from the peripheral blood of pregnant women for prenatal diagnosis of fetal aneuploides. | |
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MedLine Citation:
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PMID: 9031848 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We present the results of a study of fetal cell isolation from the peripheral blood of 46 women in the first trimester of pregnancy. The trophoblasts were sorted with paramagnetic beads labelled with a novel monoclonal antibody 340 (Mab340) (Durrant et al., Prenat. Diagn., 14 (1994) 131). This was followed by triple density gradient enrichment to remove maternal lymphocytes and red blood cells. Nucleated red blood cells (NRBC) were sorted by incubation with ferromagnetic particles coated with Mab CD71, an antitransferrin receptor monoclonal antibody, and separation on a mini-MACS column. Sorted cells were sexed using nested PCR for the Y chromosome and the results compared with the karyotypic analysis of the CVS. The sensitivity in determining a male pregnancy with NRBC alone was 38% and with trophoblasts alone was 39%. Sorting for both cell types correctly predicted a male pregnancy in 10/18 or 56%. Of the 10 males correctly identified, 3 were diagnosed on NRBC alone, 3 on trophoblast alone and 4 with both cell types. As there are very few fetal cells in maternal blood, sorting for both will increase the yield and improve diagnosis. However the technique requires further development to improve sensitivity. |
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Authors:
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L G Durrant; W L Martin; K M McDowall; D T Liu |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Early human development Volume: 47 Suppl ISSN: 0378-3782 ISO Abbreviation: Early Hum. Dev. Publication Date: 1996 Dec |
Date Detail:
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Created Date: 1997-04-30 Completed Date: 1997-04-30 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 7708381 Medline TA: Early Hum Dev Country: IRELAND |
Other Details:
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Languages: eng Pagination: S79-83 Citation Subset: IM |
Affiliation:
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Department of Clinical Oncology, University of Nottingham, City Hospital, UK. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aneuploidy* Antibodies, Monoclonal Cell Separation / methods* Erythroblasts / cytology* Female Humans Immunomagnetic Separation Male Pregnancy Prenatal Diagnosis / methods* Receptors, Transferrin / immunology Trophoblasts / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Receptors, Transferrin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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