Document Detail


Isolation of fetal trophoblast cells from peripheral blood of pregnant women.
MedLine Citation:
PMID:  1973769     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Fetal trophoblast cells were isolated from maternal peripheral blood by means of murine monoclonal antibodies of high specificity and affinity for human syncytiotrophoblast and nonvillous cytotrophoblast cells. The cells were isolated in sufficient numbers to allow polymerase chain reaction (PCR) amplification of the Y-chromosome-specific DNA sequence from the peripheral blood of thirteen pregnant women. The fetal sex predicted by PCR analysis of the isolated trophoblast cells accorded with that ascertained by karyotyping of chorionic villus samples in eleven of twelve women studied in early pregnancy and with the sex of the baby on delivery in one woman studied at 34 weeks' gestation. Isolation of these fetal cells could allow noninvasive diagnosis of a wide range of inherited disorders.
Authors:
U W Mueller; C S Hawes; A E Wright; A Petropoulos; E DeBoni; F A Firgaira; A A Morley; D R Turner; W R Jones
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Lancet     Volume:  336     ISSN:  0140-6736     ISO Abbreviation:  Lancet     Publication Date:  1990 Jul 
Date Detail:
Created Date:  1990-08-27     Completed Date:  1990-08-27     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  2985213R     Medline TA:  Lancet     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  197-200     Citation Subset:  AIM; IM    
Affiliation:
Department of Obstetrics and Gynaecology, Flinders University of South Australia, Bedford Park.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal / diagnostic use*
Cell Separation
Chorionic Villi Sampling
DNA / analysis*
Evaluation Studies as Topic
False Positive Reactions
Female
Genetic Diseases, Inborn / diagnosis
Humans
Infant, Newborn
Karyotyping
Male
Oligonucleotide Probes
Polymerase Chain Reaction
Pregnancy / blood*,  genetics
Pregnancy Trimester, First
Pregnancy Trimester, Third
Sex Determination (Analysis)
Trophoblasts / cytology*
Y Chromosome / analysis*
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Oligonucleotide Probes; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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