| Isolation of fetal nucleated red blood cell from maternal blood using immunomagnetic beads for prenatal diagnosis. | |
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MedLine Citation:
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PMID: 16913190 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The immunomagnetic beads method for isolation of fetal nucleated red blood cells (FNRBCs) from peripheral blood of 78 pregnant women for prenatal diagnosis was developed. The study subjects were classified into 8-10 and 11-14 weeks of gestation (n = 39 each). Peripheral blood cells were divided into two for the FNRBCs isolation using two protocols, one with anti-CD45 depletion followed by anti-CD71 and anti-GPA monoclonal antibodies and another without CD45 depletion. The use of CD45 depletion gave a slightly higher number of sorted cells but not significantly different (p > 0.05). The percentage of CD71+ and GPA+ cells obtained from 8-10 weeks and 11-14 weeks of gestation was not different (p > 0.05). The sensitivity in determining the sorted FNRBCs for male fetal sex by PCR using 8-10 and 11-14 weeks of gestation was generally 50 and 69%, respectively. The method so developed is simple and cost effective and may thus be applied for prenatal diagnosis. |
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Authors:
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T Pongsritasana; S Wongratanacheewin; V Prasertcharoensuk; R W Sermswan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand Volume: 24 ISSN: 0125-877X ISO Abbreviation: Asian Pac. J. Allergy Immunol. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-08-17 Completed Date: 2006-12-01 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8402034 Medline TA: Asian Pac J Allergy Immunol Country: Thailand |
Other Details:
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Languages: eng Pagination: 65-71 Citation Subset: IM |
Affiliation:
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Department of Obstetric and Gynecology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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metabolism Antigens, CD45 / metabolism Erythrocytes* Female Fetus Flow Cytometry Glycophorin / metabolism Humans Immunohistochemistry Immunomagnetic Separation* Leukocyte Reduction Procedures Polymerase Chain Reaction Pregnancy Prenatal Diagnosis / methods* Receptors, Transferrin / metabolism Sensitivity and Specificity Sex Determination (Analysis) / methods* |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/CD71 antigen; 0/GYPA protein, human; 0/Glycophorin; 0/Receptors, Transferrin; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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