| Isolation and expression of five-amino-acid-deleted variant of feline hepatocyte growth factor (HGF) cDNA. | |
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MedLine Citation:
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PMID: 14960804 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity. |
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Authors:
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Masashi Miyake; Takahiro Yaguchi; Koh-ichi Saze; Janhui Wang; Toshiyuki Ogawa; Yoshio Endo; Yasuyuki Suzuta; Masayuki Okazaki; Yoshihisa Haga; Takashi Waki; Susumu Y Takahashi; Yoshimi Yamamoto; Shigehiro Iwabuchi |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of veterinary medical science / the Japanese Society of Veterinary Science Volume: 66 ISSN: 0916-7250 ISO Abbreviation: J. Vet. Med. Sci. Publication Date: 2004 Jan |
Date Detail:
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Created Date: 2004-02-12 Completed Date: 2004-03-26 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9105360 Medline TA: J Vet Med Sci Country: Japan |
Other Details:
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Languages: eng Pagination: 9-14 Citation Subset: IM |
Affiliation:
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Central Research Laboratories, Nippon Zenyaku Kogyo Co. Ltd., Fukushima 963-0196, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cats Cell Division / drug effects Cell Line Cloning, Molecular Codon / genetics DNA Primers DNA, Complementary / genetics Dogs Hepatocyte Growth Factor / chemistry, genetics*, pharmacology Kidney Polymerase Chain Reaction Recombinant Proteins / pharmacology Sequence Deletion* |
| Chemical | |
Reg. No./Substance:
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0/Codon; 0/DNA Primers; 0/DNA, Complementary; 0/Recombinant Proteins; 67256-21-7/Hepatocyte Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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