Document Detail


Isolation of epsilon-haemoglobin-chain positive fetal cells with micromanipulation for prenatal diagnosis.
MedLine Citation:
PMID:  15906407     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVES: The isolation and analysis of fetal cells in maternal blood during pregnancy is under investigation as a means of noninvasive prenatal diagnosis. The aim of our study was to detect fetal gender from maternal peripherial blood samples during pregnancy with the detection and analysis of epsilon-haemoglobin-chain positive fetal nucleated red blood cells (NRBCs) collected by a micromanipulator. Here we report our first results. DESIGN AND METHODS: We obtained maternal blood from 14 singleton pregnancies. After a double density gradient separation, magnetic activated cell sorting was performed by positive selection for nucleated red blood cells with anti-CD71. With the help of this enrichment step, followed by immunophenotyping with an anti-haemoglobin-epsilon monoclonal antibody, the isolation of the epsilon haemoglobin chain positive cells with micromanipulation could be done. We performed single cell fluorescent PCR analysis of these cells; we used primers for the amelogenin gene to detect fetal gender. We compared our findings with the results of amniocentesis. RESULTS: Fetal gender was successfully determined in 11 out of 14 cases; among them, in 2 cases with Klinefelter syndrome (47,XXY). CONCLUSION: The results of our study suggest that micromanipulation and QF-PCR analysis of anti-haemoglobin-epsilon fluorescent antibody stained fetal cells from maternal blood can be useful in prenatal diagnosis to detect fetal gender and promising to be improved to detect chromosomal abnormalities.
Authors:
Gyula Richárd Nagy; Zoltán Bán; Ferenc Sipos; Artúr Beke; Csaba Papp; Zoltán Papp
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  25     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-26     Completed Date:  2005-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  398-402     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 John Wiley & Sons, Ltd.
Affiliation:
Department of Obstetrics and Gynecology, Semmelweis University, Faculty of Medicine, Budapest, Hungary. nagygyr@noi1.sote.hu
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MeSH Terms
Descriptor/Qualifier:
Antigens, CD / immunology*
Erythroblasts / cytology*
Female
Fetal Hemoglobin / immunology*
Fluorescent Antibody Technique
Globins / analysis*
Humans
Male
Micromanipulation
Polymerase Chain Reaction
Predictive Value of Tests
Pregnancy
Pregnancy Trimester, First
Prenatal Diagnosis*
Sex Determination (Genetics)*
Chemical
Reg. No./Substance:
0/Antigens, CD; 9004-22-2/Globins; 9034-63-3/Fetal Hemoglobin

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