Document Detail

Isolation of epsilon-haemoglobin-chain positive fetal cells with micromanipulation for prenatal diagnosis.
MedLine Citation:
PMID:  15906407     Owner:  NLM     Status:  MEDLINE    
OBJECTIVES: The isolation and analysis of fetal cells in maternal blood during pregnancy is under investigation as a means of noninvasive prenatal diagnosis. The aim of our study was to detect fetal gender from maternal peripherial blood samples during pregnancy with the detection and analysis of epsilon-haemoglobin-chain positive fetal nucleated red blood cells (NRBCs) collected by a micromanipulator. Here we report our first results. DESIGN AND METHODS: We obtained maternal blood from 14 singleton pregnancies. After a double density gradient separation, magnetic activated cell sorting was performed by positive selection for nucleated red blood cells with anti-CD71. With the help of this enrichment step, followed by immunophenotyping with an anti-haemoglobin-epsilon monoclonal antibody, the isolation of the epsilon haemoglobin chain positive cells with micromanipulation could be done. We performed single cell fluorescent PCR analysis of these cells; we used primers for the amelogenin gene to detect fetal gender. We compared our findings with the results of amniocentesis. RESULTS: Fetal gender was successfully determined in 11 out of 14 cases; among them, in 2 cases with Klinefelter syndrome (47,XXY). CONCLUSION: The results of our study suggest that micromanipulation and QF-PCR analysis of anti-haemoglobin-epsilon fluorescent antibody stained fetal cells from maternal blood can be useful in prenatal diagnosis to detect fetal gender and promising to be improved to detect chromosomal abnormalities.
Gyula Richárd Nagy; Zoltán Bán; Ferenc Sipos; Artúr Beke; Csaba Papp; Zoltán Papp
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Prenatal diagnosis     Volume:  25     ISSN:  0197-3851     ISO Abbreviation:  Prenat. Diagn.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-26     Completed Date:  2005-09-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8106540     Medline TA:  Prenat Diagn     Country:  England    
Other Details:
Languages:  eng     Pagination:  398-402     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2005 John Wiley & Sons, Ltd.
Department of Obstetrics and Gynecology, Semmelweis University, Faculty of Medicine, Budapest, Hungary.
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MeSH Terms
Antigens, CD / immunology*
Erythroblasts / cytology*
Fetal Hemoglobin / immunology*
Fluorescent Antibody Technique
Globins / analysis*
Polymerase Chain Reaction
Predictive Value of Tests
Pregnancy Trimester, First
Prenatal Diagnosis*
Sex Determination (Genetics)*
Reg. No./Substance:
0/Antigens, CD; 9004-22-2/Globins; 9034-63-3/Fetal Hemoglobin

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