Document Detail

Isolation of an episomal yeast gene and replication origin as chromatin.
MedLine Citation:
PMID:  3532106     Owner:  NLM     Status:  MEDLINE    
A multicopy yeast plasmid containing the TRP1 gene (coding for N-5'-phosphoribosylanthranilate isomerase) and ARS1 (autonomously replicating sequence 1) has been purified as chromatin. Electrophoretic analysis of nucleic acid and proteins and electron microscopy show that the plasmid chromatin is largely free of contaminants. Electron-microscopic and linking-number analyses indicate that the plasmid chromatin contains seven nucleosomes, as predicted by the indirect end-label analyses of Thoma, Bergman, and Simpson [J. Mol. Biol. (1984) 177, 715-733]. Indirect end label mapping of micrococcal nuclease cuts demonstrates that nucleosome positions and nuclease-sensitive regions are not altered by the purification. The plasmid chromatin behaves homogeneously with respect to its elution from nuclei, template activity, and intrinsic buoyant density. Taken together, these observations suggest that different copies of the TRP1ARS1 plasmid do not differ from each other grossly in chromatin structure. We discuss the potential for understanding eukaryotic gene regulation offered by the ability to isolate unique genes as chromatin.
D S Pederson; M Venkatesan; F Thoma; R T Simpson
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  83     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1986 Oct 
Date Detail:
Created Date:  1986-10-30     Completed Date:  1986-10-30     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  7206-10     Citation Subset:  IM    
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MeSH Terms
Aldose-Ketose Isomerases*
Carbohydrate Epimerases / genetics
Chromatin / isolation & purification*
DNA Replication*
DNA, Circular / isolation & purification
DNA, Fungal / isolation & purification
Genes, Fungal*
Molecular Weight
Saccharomyces cerevisiae / genetics*
Reg. No./Substance:
0/Chromatin; 0/DNA, Circular; 0/DNA, Fungal; 73-22-3/Tryptophan; EC 5.1.3.-/Carbohydrate Epimerases; EC 5.3.1/Aldose-Ketose Isomerases; EC isomerase

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