Document Detail


Isolation of egg cells and zygotes of Torenia fournieri L. and determination of their surface charge.
MedLine Citation:
PMID:  18405439     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Egg cells of Torenia fournieri were isolated from embryo sacs 1 day after anthesis using enzymatic digestion or mechanical dissection. About 5% of the egg cells and zygotes (2-3 from 50 ovules) could be mechanically dissected within 2 h. When 0.1% cellulase and 0.1% pectinase were added to the mannitol isolation solution, about 18% of the egg cells (8-10 from 50 ovules) could be isolated within 2 h. The egg cells isolated by mechanical dissection could be used for in vitro fertilization studies without any of the potentially deleterious effects of the enzymes on the plasma membrane of egg cell. The egg cells isolated using enzymatic digestion could be used in the study of the molecular biology of female gamete because more egg cells could be isolated with this technique. Using enzymatic digestion, over 10 zygotes from 50 ovules (over 20%) were isolated from the pollinated ovules. Coupled with our successful isolation of mature sperm cells, the isolation of egg cells of T. fournieri will make in vitro fertilization possible in a dicotyledon plant.
Authors:
S H Chen; Y H Yang; J P Liao; A X Kuang; H Q Tian
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zygote (Cambridge, England)     Volume:  16     ISSN:  0967-1994     ISO Abbreviation:  Zygote     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-14     Completed Date:  2008-08-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9309124     Medline TA:  Zygote     Country:  England    
Other Details:
Languages:  eng     Pagination:  179-86     Citation Subset:  IM    
Affiliation:
South China Botanical Garden, the Chinese Academy of Sciences, Guangzhou 510650, China.
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MeSH Terms
Descriptor/Qualifier:
Cell Separation
Electrophoresis
Scrophulariaceae / cytology,  physiology*
Seeds / cytology,  physiology*
Surface Properties

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