| Isolation of drugs from blood by column chromatography on Amberlite XAD-2. | |
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MedLine Citation:
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PMID: 580368 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An investigation was carried out on the isolation of 28 drugs from blood by column chromatography on Amberlite XAD-2. The following substances were added to postmortem blood specimens at concentrations generally of 1--10 microgram/ml: barbital, heptabarbital, hexobarbital, pentobarbital, phenobarbital, phenylbutazone, monocrotophos, amidopyrine, carbromal, diazepam, meprobamate, methaqualone, nitrazepam, phenazetin, chlorpromazine, dibenzepin, diphenhydramine, haloperidol, imipramine, mescaline, methadone, morphine, pentazocine, pethidine, tilidine, triflupromazine, verapamil, N-propylajmalinium bitartrate. The samples were purified by column chromatography on Amberlite XAD-2 and simple solvent extraction and subsequently quantitated by gas chromatography. By systematic variation of the conditions for adsorption of the drugs and desorption from the resin, revoveries of more than 80% after XAD-2 column chromatography could be achieved for most drugs. Thereby it was demonstrated, that in cases of fatal poisoning or emergencies this procedure is a valuable tool for forensic and clinical toxicologists who have to find out the toxic agent by chemical analysis of the blood. |
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Authors:
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H P Gelbke; T H Grell; G Schmidt |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Archives of toxicology Volume: 39 ISSN: 0340-5761 ISO Abbreviation: Arch. Toxicol. Publication Date: 1978 Jan |
Date Detail:
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Created Date: 1978-05-08 Completed Date: 1978-05-08 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0417615 Medline TA: Arch Toxicol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 211-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Gas Chromatography, Ion Exchange Humans Pharmaceutical Preparations / blood* |
| Chemical | |
Reg. No./Substance:
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0/Pharmaceutical Preparations |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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