Document Detail

Isolation and distribution of a Drosophila protein preferentially associated with inactive regions of the genome.
MedLine Citation:
PMID:  3125020     Owner:  NLM     Status:  MEDLINE    
The distribution patterns of chromosomal proteins from Drosophila can be observed by immunofluorescent staining of the polytene chromosomes from larval salivary glands. We have purified a non-histone chromosomal protein of Mr = 69,000 molecular weight which has a high affinity for DNA with little sequence specificity. Immunofluorescent staining indicates that this protein is preferentially associated with the inactive portions of the genome, including the centric heterochromatin and the condensed bands within the euchromatic arms of the chromosomes. Observation of both the heat shock loci 87A and 87C and the developmentally regulated loci 74EF and 75B shows an inverse correlation between immunofluorescent staining for the Mr = 69,000 protein and for RNA polymerase. The presence of this protein appears to be correlated with the packaging of the chromatin in an inactive form.
G Fleischmann; R Filipski; S C Elgin
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Chromosoma     Volume:  96     ISSN:  0009-5915     ISO Abbreviation:  Chromosoma     Publication Date:  1987  
Date Detail:
Created Date:  1988-04-07     Completed Date:  1988-04-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985138R     Medline TA:  Chromosoma     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  83-90     Citation Subset:  IM    
Lehrstuhl für Biochemie, Ruhr-Universität, Bochum, Federal Republic of Germany.
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MeSH Terms
Chromosomal Proteins, Non-Histone / isolation & purification*,  metabolism
Chromosomes / metabolism
DNA / metabolism
Drosophila melanogaster / genetics,  metabolism*
Molecular Weight
Reg. No./Substance:
0/Chromosomal Proteins, Non-Histone; 9007-49-2/DNA

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