Document Detail


Isolation and directed differentiation of neural crest stem cells derived from human embryonic stem cells.
MedLine Citation:
PMID:  18037878     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vertebrate neural crest development depends on pluripotent, migratory precursor cells. Although avian and murine neural crest stem (NCS) cells have been identified, the isolation of human NCS cells has remained elusive. Here we report the derivation of NCS cells from human embryonic stem cells at the neural rosette stage. We show that NCS cells plated at clonal density give rise to multiple neural crest lineages. The human NCS cells can be propagated in vitro and directed toward peripheral nervous system lineages (peripheral neurons, Schwann cells) and mesenchymal lineages (smooth muscle, adipogenic, osteogenic and chondrogenic cells). Transplantation of human NCS cells into the developing chick embryo and adult mouse hosts demonstrates survival, migration and differentiation compatible with neural crest identity. The availability of unlimited numbers of human NCS cells offers new opportunities for studies of neural crest development and for efforts to model and treat neural crest-related disorders.
Authors:
Gabsang Lee; Hyesoo Kim; Yechiel Elkabetz; George Al Shamy; Georgia Panagiotakos; Tiziano Barberi; Viviane Tabar; Lorenz Studer
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-11-25
Journal Detail:
Title:  Nature biotechnology     Volume:  25     ISSN:  1546-1696     ISO Abbreviation:  Nat. Biotechnol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-12-10     Completed Date:  2008-02-22     Revised Date:  2008-09-15    
Medline Journal Info:
Nlm Unique ID:  9604648     Medline TA:  Nat Biotechnol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1468-75     Citation Subset:  IM    
Affiliation:
Developmental Biology Program, Sloan-Kettering Institute, 1275 York Ave., New York, New York 10021, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Culture Techniques / methods
Cell Differentiation
Cell Movement
Cell Survival
Cells, Cultured
Chick Embryo
Embryonic Stem Cells / cytology*,  physiology*
Humans
Mice
Neural Crest / cytology*,  physiology*
Neurons / cytology*,  physiology*
Tissue Engineering / methods*
Comments/Corrections
Erratum In:
Nat Biotechnol. 2008 Jul;26(7):831

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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