Document Detail


Isolation of definitive zone and chromaffin cells based upon expression of CD56 (neural cell adhesion molecule) in the human fetal adrenal gland.
MedLine Citation:
PMID:  12915688     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The cortex of the human midgestation adrenals comprises a thin layer of cells, the definitive zone (DZ) that surrounds the larger, inner fetal zone (FZ). CD56 expression was observed by immunohistochemistry on DZ cells and isolated groups of cells within the FZ. CD56 mRNA expression also was detected among DZ cells but not selected sections of FZ cells isolated by laser capture microdissection. Flow cytometric analysis of dispersed adrenal cells indicated CD56 expression on a subset of adrenal cells lacking expression of hematopoietic (CD45(+) and CD235a(+)) and endothelial (CD31(+)) cell markers. The CD56(+)CD31(-)CD45(-)CD235a(-) cells were isolated by discontinuous-gradient centrifugation and fluorescence-activated cell sorting. The purified cells were enriched for DZ cells based on expression of mRNA for metallopanstimulin-1, nephroblastoma overexpressed gene, and 3-beta-hydroxysteroid dehydrogenase. P450c17 mRNA expression also was detected among a subset of CD56(+) cells consistent with expression of low levels of this protein in some DZ cells. The presence of a subpopulation of chromaffin cells among the CD56(+) population also was shown by the expression of chromogranin A mRNA. These findings indicate that CD56 expression can be used to isolate DZ and chromaffin cells to further study their functional and developmental properties.
Authors:
Marcus O Muench; Jennifer V Ratcliffe; Mikiye Nakanishi; Hitoshi Ishimoto; Robert B Jaffe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical endocrinology and metabolism     Volume:  88     ISSN:  0021-972X     ISO Abbreviation:  J. Clin. Endocrinol. Metab.     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-13     Completed Date:  2003-09-11     Revised Date:  2013-11-01    
Medline Journal Info:
Nlm Unique ID:  0375362     Medline TA:  J Clin Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  3921-30     Citation Subset:  AIM; IM    
Affiliation:
Department of Laboratory Medicine and Center for Reproductive Sciences, University of California, San Francisco, San Francisco, California 94143, USA.
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / cytology,  embryology,  metabolism*
Adult
Animals
Antigens, CD56 / biosynthesis*
Cell Differentiation
Cells, Cultured
Chromaffin Cells / metabolism*
Female
Flow Cytometry
Humans
Immunohistochemistry
Membrane Proteins / biosynthesis,  genetics
Mice
Micromanipulation
Pregnancy
RNA, Messenger / biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Grant Support
ID/Acronym/Agency:
DK59301/DK/NIDDK NIH HHS; HD08478/HD/NICHD NIH HHS; K01 DK059301/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD56; 0/Membrane Proteins; 0/RNA, Messenger

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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