| Isolation, culture and characterization of human peritoneal mesothelial cells. | |
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MedLine Citation:
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PMID: 2362409 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study establishes a reproducible technique for the culture of human peritoneal mesothelial cells. Direct explants, as well as enzymatically degraded specimens, of human omentum have been used as the source of cells. Cells were grown on collagen and gelatin coated matrices and were maintained in supplemented Ham's F-12 medium containing 10% (vol/vol) Fetal calf serum. Morphologically and ultrastructurally, the cells formed a homogeneous population. They were polygonal when confluent and devoid of contaminating fibroblasts, endothelial cells and macrophages. Cultured mesothelial cells co-expressed cytokeratin and vimentin and synthesized laminin, fibronectin, mesosecrin, non-specific esterase and collagen Types I and III but not Type IV. Ultrastructural features included numerous surface microvilli, cytoplasmic vesicles and an abundant endoplasmic reticulum. The stimulation of mesothelial cells by the calcium ionophore A23187 demonstrated that the two major products of arachidonic acid metabolism were prostacyclin and prostaglandin E2. The peritoneal mesothelial cell may be pivotal in the initiation of the inflammatory response during peritonitis and its establishment in culture will provide the basis for an in vitro model of peritoneal inflammation. |
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Authors:
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E Stylianou; L A Jenner; M Davies; G A Coles; J D Williams |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Kidney international Volume: 37 ISSN: 0085-2538 ISO Abbreviation: Kidney Int. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-08-06 Completed Date: 1990-08-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0323470 Medline TA: Kidney Int Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1563-70 Citation Subset: IM |
Affiliation:
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Institute of Nephrology, University of Wales College of Medicine, Cardiff, United Kingdom. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cells, Cultured Culture Media Humans Immunohistochemistry Microscopy, Electron Microscopy, Electron, Scanning Omentum Peritoneal Cavity / cytology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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