Document Detail


Isolation and cultivation of integrin alpha(6)beta(1)-expressing salivary gland graft cells: a model for use with an artificial salivary gland.
MedLine Citation:
PMID:  18333785     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Regeneration of the salivary glands' (SGs) normal function for patients with cancer of the head and neck treated with irradiation would be a major contribution to their quality of life. This could be accomplished by re-implantation of autologous SG cells into the residual irradiated tissue or by implantation of tissue-engineered artificial SGs. Both methods depend on the isolation of cells able to propagate and differentiate into SG epithelial cells. Recently, it has been shown that SG integrin alpha(6)beta(1)-expressing (SGIE) cells have stem cell capabilities, but these cells could be isolated only after duct ligation insult requiring surgical intervention. Because such an invasive procedure is not clinically acceptable for these patients, our aim in the present study was to explore the use of immuno-magnetic separation of untreated and short heat stress-conditioned rats as a less-insulting methodology for enhancement of these cells. Our results show that submandibular SGIE cells could be isolated and cultivated from untreated animals. However, short heat stress (HS) increased the number of isolated SGIE cells 4.7-fold and their proliferation and clonal capability 4.6-fold and 3 fold, respectively. We believe that SGIE graft cells may be suitable candidates for future tissue-engineered SGs that have been damaged by irradiation in patients with head and neck cancer.
Authors:
Ran David; Ela Shai; Doron J Aframian; Aaron Palmon
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Tissue engineering. Part A     Volume:  14     ISSN:  1937-3341     ISO Abbreviation:  -     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2008-03-12     Completed Date:  2008-07-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101466659     Medline TA:  Tissue Eng Part A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  331-7     Citation Subset:  IM    
Affiliation:
Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University-Hadassah School of Dental Medicine, Jerusalem, Israel.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Proliferation
Cell Separation / methods
Cells, Cultured
Flow Cytometry
Immunohistochemistry
Integrin alpha6beta1 / metabolism*
Male
Microscopy, Electron, Transmission
Random Allocation
Rats
Rats, Sprague-Dawley
Salivary Glands / cytology*,  metabolism*,  ultrastructure
Tissue Engineering / methods
Chemical
Reg. No./Substance:
0/Integrin alpha6beta1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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