Document Detail


Isolation and comparative mapping of a human chromosome 20-specific alpha-satellite DNA clone.
MedLine Citation:
PMID:  1733665     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated and characterized a human genomic DNA clone (PZ20, locus D20Z2) that identifies, under high-stringency hybridization conditions, an alphoid DNA subset specific for chromosome 20. The specificity was determined using fluorescence in situ hybridization. Sequence analysis confirmed our previously reported data on the great similarity between the chromosome 20 and chromosome 2 alphoid subsets. Comparative mapping of pZ20 on chimpanzee and gorilla chromosomes, also performed under high-stringency conditions, indicates that the alphoid subset has ancestral sequences on chimpanzee chromosome 11 and gorilla chromosome 19. However, no hybridization was observed to chromosomes 21 in the great apes, the homolog of human chromosome 20.
Authors:
A Baldini; N Archidiacono; R Carbone; A Bolino; V Shridhar; O J Miller; D A Miller; D C Ward; M Rocchi
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cytogenetics and cell genetics     Volume:  59     ISSN:  0301-0171     ISO Abbreviation:  Cytogenet. Cell Genet.     Publication Date:  1992  
Date Detail:
Created Date:  1992-02-28     Completed Date:  1992-02-28     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0367735     Medline TA:  Cytogenet Cell Genet     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  12-6     Citation Subset:  IM    
Affiliation:
Department of Human Genetics, Yale University School of Medicine, New Haven, CT.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Southern
Chromosome Mapping*
Chromosomes, Human, Pair 2*
Chromosomes, Human, Pair 20*
DNA, Satellite / genetics*
Fluorescence
Gorilla gorilla / genetics
Humans
Nucleic Acid Hybridization
Pan troglodytes / genetics
Grant Support
ID/Acronym/Agency:
HG00030/HG/NHGRI NIH HHS; HG00245/HG/NHGRI NIH HHS; HG00246/HG/NHGRI NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Satellite

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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