Document Detail


Isolation and chemical analysis of a lipopolysaccharide from the outer membrane of the oral anaerobic spirochete Treponema pectinovorum.
MedLine Citation:
PMID:  10551157     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Isolation of a putative lipopolysaccharide from the surface of the oral treponeme, Treponema pectinovorum, revealed it to contain larger amounts of 3-deoxy-D-manno-octulosonic acid compared with other oral Treponema species. This molecule was isolated from the outer membrane of T. pectinovorum and had chemical characteristics of a putative lipopolysaccharide. The yield of lipopolysaccharide was between 0.6% and to 1.1% of the bacterial dry weight. The purified molecule was resistant to the action of proteinases and consisted of both sugars and lipids. 3-Deoxy-D-manno-octulosonic acid and hexoses accounted for 6.1-8.7% and 17.6-20.2%, respectively of the dry weight. Carbohydrate compositional analysis revealed the presence of glucose, galactose, 2-acetamido-2-deoxy-glucose, rhamnose and 6-deoxy-talose in the molar ratio of 1.00:0.96:0.19:0.88:0.98, respectively. No heptose was detected. The fatty acid analysis determined the presence of straight chain, C13:00, C14:00, C15:00 and C17:00 acids, as well as branched chain, C13:00, C14:00 and two species of C15:00, acids. Electrophoretic analysis indicated that the lipopolysaccharide was present as two major species.
Authors:
S G Walker; X Xu; E Altman; K J Davis; J L Ebersole; S C Holt
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Oral microbiology and immunology     Volume:  14     ISSN:  0902-0055     ISO Abbreviation:  Oral Microbiol. Immunol.     Publication Date:  1999 Oct 
Date Detail:
Created Date:  1999-11-22     Completed Date:  1999-11-22     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8707451     Medline TA:  Oral Microbiol Immunol     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  304-8     Citation Subset:  D    
Affiliation:
Department of Microbiology, University of Texas Health Science Center at San Antonio 78284-7758, USA.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane / chemistry
Electrophoresis, Polyacrylamide Gel
Fatty Acids / analysis
Hexoses / analysis
Lipopolysaccharides / chemistry*,  isolation & purification
Sugar Acids / analysis
Treponema / chemistry*
Grant Support
ID/Acronym/Agency:
DE 11368/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Fatty Acids; 0/Hexoses; 0/Lipopolysaccharides; 0/Sugar Acids; 1069-03-0/2-keto-3-deoxyoctonate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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