| Isolation and characterization of a unique group of slowly growing mycobacteria: description of Mycobacterium lentiflavum sp. nov. | |
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MedLine Citation:
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PMID: 8727884 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A distinct group of slowly growing mycobacteria was identified on the basis of growth characteristics, biochemical and lipid profiles, and nucleic acid analyses. The isolates showed growth at 22 to 37 degrees C, yellow pigmentation, and negative tests for Tween 80 hydrolysis, nicotinic acid, nitrate reductase, and urease; tests for arylsulfatase, pyrazinamidase, and heat-stable catalase were variable. Analysis of cellular fatty acids by gas-liquid chromatography and mycolic acids by thin-layer chromatography and high-performance liquid chromatography indicated a distinctive pattern which was unlike those of other species. Determination of the 16S rRNA gene sequence showed a unique sequence closely related to Mycobacterium simiae and M. genavense. On the basis of DNA homology studies, we suggest that these organisms are representatives of a novel species, for which the name M. lentiflavum sp. nov. is proposed. |
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Authors:
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B Springer; W K Wu; T Bodmer; G Haase; G E Pfyffer; R M Kroppenstedt; K H Schröder; S Emler; J O Kilburn; P Kirschner; A Telenti; M B Coyle; E C Böttger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of clinical microbiology Volume: 34 ISSN: 0095-1137 ISO Abbreviation: J. Clin. Microbiol. Publication Date: 1996 May |
Date Detail:
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Created Date: 1996-10-24 Completed Date: 1996-10-24 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 7505564 Medline TA: J Clin Microbiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1100-7 Citation Subset: IM |
Affiliation:
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Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/X80769; X80770; X93995 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Typing Techniques Base Sequence DNA Primers / genetics DNA, Bacterial / genetics DNA, Ribosomal / genetics Enzymes / analysis Fatty Acids / analysis Genes, Bacterial Humans Molecular Sequence Data Mycobacterium / classification*, genetics, isolation & purification* Phylogeny RNA, Bacterial / genetics RNA, Ribosomal, 16S / genetics Sequence Homology, Nucleic Acid Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/DNA Primers; 0/DNA, Bacterial; 0/DNA, Ribosomal; 0/Enzymes; 0/Fatty Acids; 0/RNA, Bacterial; 0/RNA, Ribosomal, 16S |
| Comments/Corrections | |
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