Document Detail

Isolation and characterization of undersulphated chondroitin-4-sulphate from normal human plasma.
MedLine Citation:
PMID:  1182196     Owner:  NLM     Status:  MEDLINE    
The present study was undertaken in order to characterize further the glycosaminoglycans of normal human plasma. Coagulation factor IX concentrate prepared from undiluted plasma by DEAE-Sephadex chromatography was used as the starting material. The concentrate was subjected to proteolytic treatment with papain and pronase, deproteinised with trichloroacetic acid, dialysed and passed through an AG 1 X 2 anion-exchange column. Glycosaminoglycans were eluted stepwise from the column with NaC1. The sole glycosaminoglycan obtained was an undersulphated chondroitin-4-sulphate which was identified by chemical analyses, digestibility with testicular hyaluronidase, electrophoretic behaviour and infrared spectrum. Gel-exclusion chromatography indicated a molecular weight of 17 000 for the compound. The undersulphated chondroitin-4-sulphate was calculated to represent at least 80% of the macromolecular glycosaminoglycans present in normal human plasma and to occur in a concentration of approx. 3 mg hexuronate per 1 of plasma.
M Juvani; C Friman; H Ranta; O Wegelius
Related Documents :
25281326 - Paternal intake of folate and vitamins b6 and b12 before conception and risk of childho...
8369416 - Human olfactory neurons respond to odor stimuli with an increase in cytoplasmic ca2+.
17573366 - Reassessment of tritium dose coefficients for the general public.
10370266 - Coastal marine communities: trends and perspectives from human-exclusion experiments.
19545946 - In vitro culture combined with quantitative taqman pcr for the assessment of toxoplasma...
15927146 - Functional analysis of the ala67thr polymorphism in agouti related protein associated w...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  411     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 Nov 
Date Detail:
Created Date:  1976-01-29     Completed Date:  1976-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / analysis
Carbohydrates / analysis
Chondroitin / analogs & derivatives*
Chondroitin Sulfates / blood*,  isolation & purification
Molecular Weight
Spectrophotometry, Infrared
Sulfuric Acids / analysis
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Sulfuric Acids; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates; EC

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chemical structure of monosialoganglioside GM1b biosynthesized in vitro.
Next Document:  Beta-Elimination and sulfite addition reaction of chondroitin sulfate peptidoglycan and the peptide ...