| Isolation and characterization of undersulphated chondroitin-4-sulphate from normal human plasma. | |
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MedLine Citation:
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PMID: 1182196 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The present study was undertaken in order to characterize further the glycosaminoglycans of normal human plasma. Coagulation factor IX concentrate prepared from undiluted plasma by DEAE-Sephadex chromatography was used as the starting material. The concentrate was subjected to proteolytic treatment with papain and pronase, deproteinised with trichloroacetic acid, dialysed and passed through an AG 1 X 2 anion-exchange column. Glycosaminoglycans were eluted stepwise from the column with NaC1. The sole glycosaminoglycan obtained was an undersulphated chondroitin-4-sulphate which was identified by chemical analyses, digestibility with testicular hyaluronidase, electrophoretic behaviour and infrared spectrum. Gel-exclusion chromatography indicated a molecular weight of 17 000 for the compound. The undersulphated chondroitin-4-sulphate was calculated to represent at least 80% of the macromolecular glycosaminoglycans present in normal human plasma and to occur in a concentration of approx. 3 mg hexuronate per 1 of plasma. |
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Authors:
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M Juvani; C Friman; H Ranta; O Wegelius |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 411 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1975 Nov |
Date Detail:
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Created Date: 1976-01-29 Completed Date: 1976-01-29 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-10 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Carbohydrates / analysis Chondroitin / analogs & derivatives* Chondroitin Sulfates / blood*, isolation & purification Humans Hyaluronoglucosaminidase Molecular Weight Spectrophotometry, Infrared Sulfuric Acids / analysis |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Carbohydrates; 0/Sulfuric Acids; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates; EC 3.2.1.35/Hyaluronoglucosaminidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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