Document Detail


Isolation and characterization of undersulphated chondroitin-4-sulphate from normal human plasma.
MedLine Citation:
PMID:  1182196     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The present study was undertaken in order to characterize further the glycosaminoglycans of normal human plasma. Coagulation factor IX concentrate prepared from undiluted plasma by DEAE-Sephadex chromatography was used as the starting material. The concentrate was subjected to proteolytic treatment with papain and pronase, deproteinised with trichloroacetic acid, dialysed and passed through an AG 1 X 2 anion-exchange column. Glycosaminoglycans were eluted stepwise from the column with NaC1. The sole glycosaminoglycan obtained was an undersulphated chondroitin-4-sulphate which was identified by chemical analyses, digestibility with testicular hyaluronidase, electrophoretic behaviour and infrared spectrum. Gel-exclusion chromatography indicated a molecular weight of 17 000 for the compound. The undersulphated chondroitin-4-sulphate was calculated to represent at least 80% of the macromolecular glycosaminoglycans present in normal human plasma and to occur in a concentration of approx. 3 mg hexuronate per 1 of plasma.
Authors:
M Juvani; C Friman; H Ranta; O Wegelius
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  411     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1975 Nov 
Date Detail:
Created Date:  1976-01-29     Completed Date:  1976-01-29     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  1-10     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Amino Acids / analysis
Carbohydrates / analysis
Chondroitin / analogs & derivatives*
Chondroitin Sulfates / blood*,  isolation & purification
Humans
Hyaluronoglucosaminidase
Molecular Weight
Spectrophotometry, Infrared
Sulfuric Acids / analysis
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Carbohydrates; 0/Sulfuric Acids; 9007-27-6/Chondroitin; 9007-28-7/Chondroitin Sulfates; EC 3.2.1.35/Hyaluronoglucosaminidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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