Document Detail

Isolation and characterization of two strains of Fusarium oxysporum causing potato dry rot in Solanum tuberosum in Colombia.
MedLine Citation:
PMID:  21635960     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Fusarium oxysporum has worldwide distribution and causes severe vascular wilt or root rot in many plants. Strains are classified into formae speciales based on their high degree of host specificity, of which multilocus sequence typing provides a fairly good estimate.
AIMS: The main aim of this study was to identify the causal agent of an infected potato tuber in Colombia.
METHODS: Two F. oxysporum isolates were recovered from a potato tuber showing symptoms of dry rot. Both macroscopic and microscopic morphology differences were observed between the two isolates. Koch's postulates were verified and in quantitative tuber pathogenecity trials, both isolates induced moderate dry rot. Ribosomal internal transcribed spacer (ITS) and partial intergenic spacer region (IGS) sequences were PCR-amplified, sequenced and shown to be identical for the two isolates. A maximum parsimony phylogeny was created using F. oxysporum IGS sequences available in the Genebank database, which does not include sequences from the formae speciales tuberosi.
RESULTS: Our two isolates were most closely related to a red clover (Trifolium pratense) pathogenic isolate and two non-pathogenic F. oxysporum isolates from birdsfoot trefoil (Lotus corniculatus) and Lycopersicon sp. rhyzosphere (99% identity).
CONCLUSIONS: These experiments showed that our isolates are not restricted to potato and that a molecular marker is needed to differentiate the formae speciales since the IGS and EF-1α do not have the power to do it.
Leonor García Bayona; Alejandro Grajales; Martha Emiliana Cárdenas; Roberto Sierra; Gabriel Lozano; Manuel Fernando Garavito; María Caridad Cepero de García; Adriana Bernal; Pedro Jiménez; Silvia Restrepo
Publication Detail:
Type:  Journal Article     Date:  2011-05-04
Journal Detail:
Title:  Revista iberoamericana de micología     Volume:  28     ISSN:  2173-9188     ISO Abbreviation:  Rev Iberoam Micol     Publication Date:    2011 Oct-Dec
Date Detail:
Created Date:  2011-09-30     Completed Date:  2012-02-24     Revised Date:  2012-08-31    
Medline Journal Info:
Nlm Unique ID:  9425531     Medline TA:  Rev Iberoam Micol     Country:  Spain    
Other Details:
Languages:  eng     Pagination:  166-72     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Revista Iberoamericana de Micología. Published by Elsevier Espana. All rights reserved.
Laboratorio de Micología y Fitopatología LAMFU, Universidad de los Andes, Bogotá, Colombia.
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MeSH Terms
Fusarium / isolation & purification*
Plant Diseases / microbiology*
Solanum tuberosum / microbiology*

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