Document Detail

Isolation and characterization of two pigment-dispersing hormones from the whiteleg shrimp, Litopenaeus vannamei.
MedLine Citation:
PMID:  16908959     Owner:  NLM     Status:  MEDLINE    
In crustaceans, the pigment-dispersing hormone (PDH) is released from the X-organ/sinus gland complex located in the eyestalks, and controls pigment dispersion in the chromatophores. Knowledge concerning the structure and activity of PDH in penaeid shrimps is remains limited, since natural PDH has been purified from only the Kuruma prawn, Marsupenaeus japonicus. In this study, two PDHs (Liv-PDH-A and -B) were purified from the sinus gland extracts of another penaeid species, the whiteleg shrimp, Litopenaeus vannamei, by two steps of reversed-phase HPLC, and their amino acid sequences were determined. They both consist of 18 amino acid residues, with a free N-terminus and an amidated C-terminus, the sequences of Liv-PDH-A and -B being NSELINSLLGIPKVMNDAamide and NSELINSLLGLPKVMNDAamide, respectively. These sequences are identical to those of mature PDHs deduced from cDNAs encoding L. vannamei PDH precursors cloned previously by other workers. Liv-PDH-A and -B showed significant pigment-dispersing activity in melanophores by in vivo bioassay.
Tsuyoshi Ohira; Naoaki Tsutsui; Ichiro Kawazoe; Marcy N Wilder
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoological science     Volume:  23     ISSN:  0289-0003     ISO Abbreviation:  Zool. Sci.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-15     Completed Date:  2007-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8702287     Medline TA:  Zoolog Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  601-6     Citation Subset:  IM    
Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
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MeSH Terms
Amino Acid Sequence
Chromatography, High Pressure Liquid / methods,  veterinary
DNA, Complementary / genetics
Molecular Sequence Data
Penaeidae / metabolism*
Peptides / chemistry*,  isolation & purification
Reg. No./Substance:
0/DNA, Complementary; 0/Peptides; 9046-72-4/melanophore-dispersing hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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