Document Detail


Isolation and characterization of two pigment-dispersing hormones from the whiteleg shrimp, Litopenaeus vannamei.
MedLine Citation:
PMID:  16908959     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In crustaceans, the pigment-dispersing hormone (PDH) is released from the X-organ/sinus gland complex located in the eyestalks, and controls pigment dispersion in the chromatophores. Knowledge concerning the structure and activity of PDH in penaeid shrimps is remains limited, since natural PDH has been purified from only the Kuruma prawn, Marsupenaeus japonicus. In this study, two PDHs (Liv-PDH-A and -B) were purified from the sinus gland extracts of another penaeid species, the whiteleg shrimp, Litopenaeus vannamei, by two steps of reversed-phase HPLC, and their amino acid sequences were determined. They both consist of 18 amino acid residues, with a free N-terminus and an amidated C-terminus, the sequences of Liv-PDH-A and -B being NSELINSLLGIPKVMNDAamide and NSELINSLLGLPKVMNDAamide, respectively. These sequences are identical to those of mature PDHs deduced from cDNAs encoding L. vannamei PDH precursors cloned previously by other workers. Liv-PDH-A and -B showed significant pigment-dispersing activity in melanophores by in vivo bioassay.
Authors:
Tsuyoshi Ohira; Naoaki Tsutsui; Ichiro Kawazoe; Marcy N Wilder
Related Documents :
610279 - Amino acid sequence of the basic trypsin inhibitor from bovine splenic capsule. a new f...
2103939 - Cellular differentiation in the process of generation of the eukaryotic cell.
10987369 - Characterization of snp, a novel tissue- and phase-specific nuclear protein expressed d...
3002799 - Complete amino acid sequence of rat l-type pyruvate kinase deduced from the cdna sequence.
11170559 - Identification of amino acids in wines by one- and two-dimensional nuclear magnetic res...
9339549 - Purification and characterization of ferredoxin-sulfite reductase from turnip (brassica...
16732469 - Effects of arachidonic acid on the lysosomal ion permeability and osmotic stability.
610279 - Amino acid sequence of the basic trypsin inhibitor from bovine splenic capsule. a new f...
22220589 - Bioactive polyphenols in leaves, stems and berries of saskatoon (amelanchier alnifolia ...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Zoological science     Volume:  23     ISSN:  0289-0003     ISO Abbreviation:  Zool. Sci.     Publication Date:  2006 Jul 
Date Detail:
Created Date:  2006-08-15     Completed Date:  2007-07-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8702287     Medline TA:  Zoolog Sci     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  601-6     Citation Subset:  IM    
Affiliation:
Japan International Research Center for Agricultural Sciences, Tsukuba, Ibaraki, Japan.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Animals
Chromatography, High Pressure Liquid / methods,  veterinary
DNA, Complementary / genetics
Female
Male
Molecular Sequence Data
Penaeidae / metabolism*
Peptides / chemistry*,  isolation & purification
Chemical
Reg. No./Substance:
0/DNA, Complementary; 0/Peptides; 9046-72-4/melanophore-dispersing hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Occurrence of deformed fish and their fitness-related traits in Kirikuchi charr, Salvelinus leucomae...
Next Document:  Molecular characterization of the interferon-tau gene of the mithun (Bos frontalis).