Document Detail

Isolation and characterization of tumorigenic extrahepatic cholangiocarcinoma cells with stem cell-like properties.
MedLine Citation:
PMID:  20232394     Owner:  NLM     Status:  MEDLINE    
Recent studies suggest that the ability to form and grow tumors specifically resides in a small cell population called cancer stem cells (CSCs). These studies were conducted mainly on various human cancers; however, isolation and characterization of stem cells from cholangiocarcinoma have not been attempted. The molecular markers CD24, CD44, CD34, and epithelial cell adhesion molecule (EpCAM) are widely used, individually or in combination, to characterize some types of CSCs. In this study, we used these markers to identify a subpopulation of cells in extrahepatic cholangiocarcinoma (ECC) with cancer stem/progenitor cell-like properties. We found that CD24(+) CD44(+) EpCAM(high) cells (0.39-2.27%) were present in human ECC tissues. The expression of a CD24(+) CD44(+) EpCAM(high) subpopulation was consistent with primary cancers and could be duplicated during serial in vivo passaging in NOD/SCID mice. CD24(+) CD44(+) EpCAM(high) cells isolated from 3 cholangiocarcinoma xenografts showed high tumorigenic potential compared with CD24(-) CD44(-) EpCAM(low/-) cells. These tumorigenic ECC cells exhibited the stem cell properties of self-renewal and ability to produce heterogeneous progeny. We report the identification of a CSC population in ECC characterized by CD24, CD44 and EpCAM phenotypes. Our findings could provide new insight into the tumorigenesis of cholangiocarcinoma and offer a potential target for anti-cancer therapy.
Min Wang; Juan Xiao; Min Shen; Yu Yahong; Rui Tian; Feng Zhu; Jianxin Jiang; Zhiyong Du; Jun Hu; Wensong Liu; Renyi Qin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  128     ISSN:  1097-0215     ISO Abbreviation:  Int. J. Cancer     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-10-28     Completed Date:  2010-12-30     Revised Date:  2011-03-29    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  72-81     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 UICC.
Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan City, Hubei Province, People's Republic of China.
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MeSH Terms
Antigens, CD24 / metabolism
Antigens, CD44 / metabolism
Antigens, Neoplasm / metabolism
Bile Duct Neoplasms / metabolism,  pathology*
Bile Ducts, Extrahepatic*
Cell Adhesion Molecules / metabolism
Cell Line, Tumor
Cholangiocarcinoma / metabolism,  pathology*
Flow Cytometry
Mice, Inbred NOD
Mice, SCID
Middle Aged
Neoplastic Stem Cells / metabolism,  pathology*
Transplantation, Heterologous
Tumor Markers, Biological / metabolism
Reg. No./Substance:
0/Antigens, CD24; 0/Antigens, CD44; 0/Antigens, Neoplasm; 0/Cell Adhesion Molecules; 0/Tumor Markers, Biological; 0/tumor-associated antigen GA733
Comment In:
Int J Cancer. 2011 Apr 15;128(8):1997-8   [PMID:  20568108 ]

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