| Isolation and characterization of a trypsin inhibitor from the seeds of kohlrabi (Brassica napus var. rapifera) belonging to the napin family of storage proteins. | |
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MedLine Citation:
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PMID: 2490369 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A trypsin inhibitor with a Km of 5 x 10(-5) M has been isolated from kohlrabi (Brassica napus var. rapifera). Subtilisin DY is inhibited only weakly and chymotrypsin not at all. The inhibitor is closely related to napin as determined by amino acid sequence analysis which also showed the inhibitor to be polymorphous. The inhibitor has been further characterized by means of molecular weight determination using SDS gel-electrophoresis and by amino acid analysis, fluorimetry as well as circular dichroism. A simplified method for purification of napins is given. |
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Authors:
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I B Svendsen; D Nicolova; I Goshev; N Genov |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Carlsberg research communications Volume: 54 ISSN: 0105-1938 ISO Abbreviation: Carlsberg Res. Commun. Publication Date: 1989 |
Date Detail:
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Created Date: 1991-08-06 Completed Date: 1991-08-06 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7703861 Medline TA: Carlsberg Res Commun Country: DENMARK |
Other Details:
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Languages: eng Pagination: 231-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Carlsberg Laboratory, Copenhagen Valby. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2S Albumins, Plant Amino Acid Sequence Brassica / enzymology* Chymotrypsin / antagonists & inhibitors Molecular Sequence Data Plant Proteins / genetics, isolation & purification*, metabolism Sequence Homology, Nucleic Acid Subtilisins / antagonists & inhibitors Trypsin Inhibitors / genetics, isolation & purification*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/2S Albumins, Plant; 0/Plant Proteins; 0/Trypsin Inhibitors; 89147-51-3/napin protein, Brassica napus; 9035-81-8/trypsin inhibitor protein, Brassica napus; EC 3.4.21.-/Subtilisins; EC 3.4.21.1/Chymotrypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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