| Isolation and characterization of true mesenchymal stem cells derived from human term decidua capable of multilineage differentiation into all 3 embryonic layers. | |
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MedLine Citation:
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PMID: 20692642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: The objective of the study was to isolate and characterize a population of mesenchymal stem cells (MSCs) from human term placental membranes. STUDY DESIGN: We isolated an adherent cell population from extraembryonic membranes. Morphology, phenotype, growth characteristics, karyotype, and immunological and differentiation properties were analyzed. RESULTS: The isolated placental MSCs were from maternal origin and named as decidua-derived mesenchymal stem cells (DMSCs). DMSCs differentiated into derivatives of all germ layers. It is the first report about placental MSC differentiation into alveolar type II cells. Clonally expanded DMSCs differentiated into all embryonic layers, including pulmonary cells. DMSCs showed higher life span than placental cells from fetal origin and proliferated without genomic instability. CONCLUSION: The data suggest that DMSCs are true multipotent MSCs, distinguishing them from other placental MSCs. DMSCs could be safely used in the mother as a potential source of MSCs for pelvic floor dysfunctions and immunological diseases. Additionally, frozen DMSCs can be stored for both autologous and allogeneic tissue regeneration. |
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Authors:
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Maria I Macias; Jesús Grande; Ana Moreno; Irene Domínguez; Rafael Bornstein; Ana I Flores |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-08-08 |
Journal Detail:
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Title: American journal of obstetrics and gynecology Volume: 203 ISSN: 1097-6868 ISO Abbreviation: Am. J. Obstet. Gynecol. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-08 Completed Date: 2010-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0370476 Medline TA: Am J Obstet Gynecol Country: United States |
Other Details:
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Languages: eng Pagination: 495.e9-495.e23 Citation Subset: AIM; IM |
Copyright Information:
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Copyright © 2010 Mosby, Inc. All rights reserved. |
Affiliation:
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Research Center, Hospital 12 de Octubre, Avda Cordoba s/n, Madrid, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD / metabolism Cell Differentiation* Cell Lineage* Cells, Cultured Decidua / cytology*, metabolism Female Humans Immunohistochemistry Immunophenotyping In Situ Hybridization, Fluorescence Karyotyping Mesenchymal Stem Cells / cytology*, metabolism Microsatellite Repeats Multipotent Stem Cells / cytology*, metabolism Reverse Transcriptase Polymerase Chain Reaction Telomerase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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