Document Detail


Isolation and characterization of transducing bacteriophage BP1 for Bacterium anitratum (Achromobacter sp.).
MedLine Citation:
PMID:  5723528     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A small transducing phage has been isolated against a strain of Bacterium anitratum. The particle has a head dimension of 450 A and a tail approximately 200 A long. The latent period is 16 min and the average burst size is 98. The intact particle has an absorption maximum and minimum at 260 and 237 mmu, respectively. The sedimentation coefficient (S(20)) is 460. The phage contains double-stranded DNA with an S degrees (20,w) of 32.8. Molecular weight estimates of the deoxyribonucleic acid ranged from 2.33 x 10(7) to 2.66 x 10(7) based on sedimentation velocity studies. The percentage guanine plus cytosine compositions of the deoxyribonucleic acid, determined by melting temperature and cesium chloride equilibrium centrifugation, were 40.7 and 42.0, respectively.
Authors:
R Twarog; L E Blouse
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of virology     Volume:  2     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1968 Jul 
Date Detail:
Created Date:  1969-01-25     Completed Date:  1969-01-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  716-22     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Bacteria*
Bacteriophages / growth & development,  isolation & purification*
Centrifugation, Density Gradient
Cytosine / analysis
DNA, Viral / analysis
Guanine / analysis
Hot Temperature
Microscopy, Electron
Molecular Weight
Spectrophotometry
Transduction, Genetic
Virus Replication
Chemical
Reg. No./Substance:
0/DNA, Viral; 71-30-7/Cytosine; 73-40-5/Guanine
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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