Document Detail


Isolation and characterization of terpene synthases potentially involved in flavor development of ripening olive (Olea europaea) fruits.
MedLine Citation:
PMID:  22475500     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
The flavor and taste of fruits are often determined by terpenes. We identified three cDNAs encoding putative terpene synthases from olive fruits of cv. Frantoio and Grignano. Heterologous expression in a bacterial system demonstrated that one of the terpene synthases, OeGES1, was an active monoterpene synthase that converted geranyl diphosphate to the monoterpene alcohol geraniol. The transcript accumulation pattern of this gene showed a peak during fruit ripening in both genotypes, indicating that the enzyme may be involved in the production of monoterpene flavor compounds in olive fruit. Although the putative terpene synthases OeTPS2 and OeTPS3 clustered with α-farnesene synthases and angiosperm monoterpene synthases, no detectable in vitro activity was found after expression in a bacterial system. Nevertheless, their transcripts sharply accumulated during fruit ripening starting from véraison.
Authors:
Alice Vezzaro; Sandra T Krause; Alberto Nonis; Angelo Ramina; Jörg Degenhardt; Benedetto Ruperti
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-2
Journal Detail:
Title:  Journal of plant physiology     Volume:  -     ISSN:  1618-1328     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9882059     Medline TA:  J Plant Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2012. Published by Elsevier GmbH.
Affiliation:
Department of Environmental Agronomy and Crop Science, University of Padova, Viale dell'Università 16, 35020 Legnaro (PD), Italy.
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