| Isolation and characterization of the sulfated neolactotetraosylceramide from hog gastric mucosa. | |
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MedLine Citation:
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PMID: 649586 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A sulfated neolactotetraosylceramide has been isolated from the lipid extract of hog gastric mucosa. Isolation procedure involved extraction of lipids with 0.4 M sodium acetate in methanol/chloroform/water, alkaline methanolysis, column fractionation on DEAE-Sephadex, separation of the acetylated acidic glycolipids on silicic acid column, and thin layer chromatography. By partial acid hydrolysis, sequential hydrolysis with specific glycosidases, periodate oxidation, and methylation analysis of the native and desulfated compound, the structure of this glycolipid is proposed to be: Galbeta1 leads to 4GlcNAc(6 comes from SO3)beta1 leads to 3Galbeta1 leads to 4Glc1 leads to 1 ceramide. |
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Authors:
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B L Slomiany; A Slomiany |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 253 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1978 May |
Date Detail:
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Created Date: 1978-07-24 Completed Date: 1978-07-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 3517-20 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carbohydrates / analysis Fatty Acids / analysis Gastric Mucosa / analysis* Sulfoglycosphingolipids* / isolation & purification Swine |
| Chemical | |
Reg. No./Substance:
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0/Carbohydrates; 0/Fatty Acids; 0/Sulfoglycosphingolipids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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