Document Detail


Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue.
MedLine Citation:
PMID:  23777308     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The emerging field of tissue engineering and regenerative medicine is a multidisciplinary science that is based on the combination of a reliable source of stem cells, biomaterial scaffolds, and cytokine growth factors. Adult mesenchymal stem cells are considered important cells for applications in this field, and adipose tissue has revealed to be an excellent source of them. Indeed, adipose-derived stem cells (ASCs) can be easily isolated from the stromal vascular fraction (SVF) of adipose tissue. During the isolation and propagation of murine ASCs, we observed the appearance of a spontaneously immortalized cell clone, named m17.ASC. This clone has been propagated for more than 180 passages and stably expresses a variety of stemness markers, such as Sca-1, c-kit/CD117, CD44, CD106, islet-1, nestin, and nucleostemin. Furthermore, these cells can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. m17.ASC clone displays a normal karyotype and stable telomeres; it neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-γ (null) mice. The analysis of gene expression highlighted transcriptional traits of SVF cells. m17.ASCs were genetically modified by lentiviral vectors carrying green fluorescent protein (GFP) as a marker transgene and efficiently engrafted in the liver, when injected in the spleen of NOD/SCID-γ (null) monocrotaline-treated mice. These results suggest that this non-tumorigenic spontaneously immortalized ASC line may represent a useful tool (cell model) for studying the differentiation mechanisms involved in tissue repair as well as a model for pharmacological/toxicological studies.
Authors:
Andrea Zamperone; Stefano Pietronave; Simone Merlin; Donato Colangelo; Gabriella Ranaldo; Enzo Medico; Federica Di Scipio; Giovanni Nicolao Berta; Antonia Follenzi; Maria Prat
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-08-09
Journal Detail:
Title:  Stem cells and development     Volume:  22     ISSN:  1557-8534     ISO Abbreviation:  Stem Cells Dev.     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-16     Completed Date:  2014-05-12     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  101197107     Medline TA:  Stem Cells Dev     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2873-84     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipocytes / cytology,  metabolism
Animals
Antigens, Ly / genetics,  metabolism
Cell Differentiation / genetics
Cell Line
Chondrocytes / cytology,  metabolism
Clone Cells / cytology,  metabolism,  transplantation
Gene Expression Profiling
Green Fluorescent Proteins / genetics,  metabolism
Interleukin Receptor Common gamma Subunit / deficiency,  genetics
Karyotype
Male
Membrane Proteins / genetics,  metabolism
Mesenchymal Stem Cell Transplantation / methods*
Mesenchymal Stromal Cells / cytology*,  metabolism
Mice
Mice, Inbred NOD
Mice, Knockout
Mice, SCID
Microscopy, Confocal
Multipotent Stem Cells / cytology*,  metabolism
Myocytes, Cardiac / cytology,  metabolism
Osteocytes / cytology,  metabolism
Proto-Oncogene Proteins c-kit / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Subcutaneous Fat / cytology*
Grant Support
ID/Acronym/Agency:
GGP09280//Telethon
Chemical
Reg. No./Substance:
0/Antigens, Ly; 0/Il2rg protein, mouse; 0/Interleukin Receptor Common gamma Subunit; 0/Ly6a protein, mouse; 0/Membrane Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.10.1/Proto-Oncogene Proteins c-kit
Comments/Corrections

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