| Isolation and characterization of a spontaneously immortalized multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue. | |
MedLine Citation:
|
PMID: 23777308 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The emerging field of tissue engineering and regenerative medicine is a multidisciplinary science that is based on the combination of a reliable source of stem cells, biomaterial scaffolds, and cytokine growth factors. Adult mesenchymal stem cells are considered important cells for applications in this field, and adipose tissue has revealed to be an excellent source of them. Indeed, adipose-derived stem cells (ASCs) can be easily isolated from the stromal vascular fraction (SVF) of adipose tissue. During the isolation and propagation of murine ASCs, we observed the appearance of a spontaneously immortalized cell clone, named m17.ASC. This clone has been propagated for more than 180 passages and stably expresses a variety of stemness markers, such as Sca-1, c-kit/CD117, CD44, CD106, islet-1, nestin, and nucleostemin. Furthermore, these cells can be induced to differentiate toward osteogenic, chondrogenic, adipogenic, and cardiogenic phenotypes. m17.ASC clone displays a normal karyotype and stable telomeres; it neither proliferates when plated in soft agar nor gives rise to tumors when injected subcutaneously in NOD/SCID-γ (null) mice. The analysis of gene expression highlighted transcriptional traits of SVF cells. m17.ASCs were genetically modified by lentiviral vectors carrying green fluorescent protein (GFP) as a marker transgene and efficiently engrafted in the liver, when injected in the spleen of NOD/SCID-γ (null) monocrotaline-treated mice. These results suggest that this non-tumorigenic spontaneously immortalized ASC line may represent a useful tool (cell model) for studying the differentiation mechanisms involved in tissue repair as well as a model for pharmacological/toxicological studies. |
Authors:
|
Andrea Zamperone; Stefano Pietronave; Simone Merlin; Donato Colangelo; Gabriella Ranaldo; Enzo Medico; Federica Di Scipio; Giovanni Nicolao Berta; Antonia Follenzi; Maria Prat |
Related Documents
:
|
17293268 - Cell therapy in myocardial infarction. 23225888 - Cutting edge: ly9 (cd229), a slam family receptor, negatively regulates the development... 24001408 - Enhanced dopamine release by mesenchymal stem cells reprogrammed neuronally by the modu... 14578098 - Adipose-derived adult stem cells: isolation, characterization, and differentiation pote... 19574478 - "self"-reflection by kir. 2572368 - Peripheral lymphocyte subsets and their responsiveness in human strongyloidiasis. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2013-08-09 |
Journal Detail:
|
Title: Stem cells and development Volume: 22 ISSN: 1557-8534 ISO Abbreviation: Stem Cells Dev. Publication Date: 2013 Nov |
Date Detail:
|
Created Date: 2013-10-16 Completed Date: 2014-05-12 Revised Date: 2014-11-04 |
Medline Journal Info:
|
Nlm Unique ID: 101197107 Medline TA: Stem Cells Dev Country: United States |
Other Details:
|
Languages: eng Pagination: 2873-84 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipocytes
/
cytology,
metabolism Animals Antigens, Ly / genetics, metabolism Cell Differentiation / genetics Cell Line Chondrocytes / cytology, metabolism Clone Cells / cytology, metabolism, transplantation Gene Expression Profiling Green Fluorescent Proteins / genetics, metabolism Interleukin Receptor Common gamma Subunit / deficiency, genetics Karyotype Male Membrane Proteins / genetics, metabolism Mesenchymal Stem Cell Transplantation / methods* Mesenchymal Stromal Cells / cytology*, metabolism Mice Mice, Inbred NOD Mice, Knockout Mice, SCID Microscopy, Confocal Multipotent Stem Cells / cytology*, metabolism Myocytes, Cardiac / cytology, metabolism Osteocytes / cytology, metabolism Proto-Oncogene Proteins c-kit / genetics, metabolism Reverse Transcriptase Polymerase Chain Reaction Subcutaneous Fat / cytology* |
| Grant Support | |
ID/Acronym/Agency:
|
GGP09280//Telethon |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Ly; 0/Il2rg protein, mouse; 0/Interleukin Receptor Common gamma Subunit; 0/Ly6a protein, mouse; 0/Membrane Proteins; 147336-22-9/Green Fluorescent Proteins; EC 2.7.10.1/Proto-Oncogene Proteins c-kit |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Comment on "Extending Applicability of Correlation Equations to Predict Colloidal Retention in Porou...
Next Document: Effect of black tea extract on herpes simplex virus-1 infection of cultured cells.