| Isolation and characterization of six human hepatic isometallothioneins. | |
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MedLine Citation:
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PMID: 4062903 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Human hepatic metallothionein (MT) was separated into six isoforms by using reversed-phase h.p.l.c. at the analytical and preparative levels. By comparison with the h.p.l.c. elution profiles of the charge-separable species MT-1 and MT-2 isolated by the procedure of Bühler & Kägi [(1974) FEBS Lett. 39, 229-234], five of these isoproteins are identified as hitherto unresolved subforms of MT-1, and one is identical with MT-2. The six isoforms have distinct and reproducible retention times at neutral pH, where the metal remains bound to the protein, and at low pH, where the metal is removed. Their amino acid compositions display the high cysteine content and the lack of aromatic amino acids and of histidine typical of mammalian metallothioneins, but they differ significantly with respect to all other amino acids. A survey of autopsy material indicates that in adult human liver all six isoforms are usually expressed, albeit in somewhat variable relative proportions. |
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Authors:
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P E Hunziker; J H Kägi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Biochemical journal Volume: 231 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1985 Oct |
Date Detail:
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Created Date: 1985-12-19 Completed Date: 1985-12-19 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 375-82 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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analysis Chromatography, High Pressure Liquid Chromatography, Ion Exchange Humans Liver / analysis* Metallothionein / isolation & purification* |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 9038-94-2/Metallothionein |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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