Document Detail


Isolation and characterization of sea urchin egg spectrin: calcium modulation of the spectrin-actin interaction.
MedLine Citation:
PMID:  3607894     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sea urchin egg spectrin has been purified from a homogenate of unfertilized Strongylocentrotus purpuratus eggs using standard biochemical procedures. SDS-PAGE analysis of the molecule revealed a closely spaced, high molecular weight doublet at 237/234 kDa (present in an equimolar ratio). Rotary shadowed images of egg spectrin revealed a double-stranded, elongate, flexible rod-shaped contour, measuring 210 nm in length and approximately 4-8 nm in width. Additionally, this molecule is shown to be immunologically related to avian erythroid spectrin, since it crossreacts with antibodies prepared against the chicken erythrocyte alpha-spectrin/240 kDa subunit. The interaction of egg spectrin with actin was examined by sedimentation and falling-ball viscometry assays. The binding and cross linking properties of spectrin to actin demonstrate a unique Ca++-sensitive regulation at micromolar Ca++ concentrations. This observation provides new insight into the way Ca++ may regulate spectrin-actin interactions in vitro and further suggests possible structural and modulatory roles for egg spectrin in the developing sea urchin embryo.
Authors:
D J Fishkind; E M Bonder; D A Begg
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Cell motility and the cytoskeleton     Volume:  7     ISSN:  0886-1544     ISO Abbreviation:  Cell Motil. Cytoskeleton     Publication Date:  1987  
Date Detail:
Created Date:  1987-09-14     Completed Date:  1987-09-14     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8605339     Medline TA:  Cell Motil Cytoskeleton     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  304-14     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Calcium / pharmacology*
Cytoskeleton / metabolism
Female
Molecular Weight
Ovum / metabolism*
Sea Urchins
Spectrin / isolation & purification,  metabolism*
Viscosity
Grant Support
ID/Acronym/Agency:
GM 07226/GM/NIGMS NIH HHS; GM 07258/GM/NIGMS NIH HHS; GM 09788/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 12634-43-4/Spectrin; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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