| Isolation and characterization of the reaction product of 4-diazobenzenesulfonic acid and gamma-carboxyglutamic acid: modification of the assay for measurement of beta-carboxyaspartic acid. | |
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MedLine Citation:
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PMID: 8135347 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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gamma-Carboxyglutamic acid (Gla)-containing proteins are extracellular proteins with enhanced cation or mineral-binding properties. Discovery of new Gla-containing proteins is facilitated by methods that decrease the number of steps and time involved in assaying for Gla. Reaction of 4-diazobenzenesulfonic acid (DBS) and Gla or Gla-containing proteins produces an intensely red-colored product. This method has been used to identify Gla-containing proteins in crude extracts of proteins. The reaction product of Gla and DBS has been purified by reversed-phase HPLC and characterized by uv-visible spectroscopy and electrospray ionization (ESI) mass spectrometry (MS). The red-colored product exhibits an absorption maximum at 530 nm. The ESI-MS data of the colored derivative of Gla are consistent with replacement of the two gamma-carboxyl groups in Gla with two DBS groups. DBS also reacts with another malonic acid derivative, beta-carboxyaspartic acid (Asa). The optimum conditions for colorimetric assay of Asa were established, and the potential of this reaction as an assay for Asa and Asa-containing proteins was studied. |
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Authors:
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S K Nishimoto; J Zhao; C Dass |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 216 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-04-18 Completed Date: 1994-04-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 159-64 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, University of Tennessee, Memphis 38163. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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1-Carboxyglutamic Acid* Aspartic Acid / analogs & derivatives*, analysis Chromatography, High Pressure Liquid Colorimetry Diazonium Compounds* Mass Spectrometry Sulfanilic Acids* |
| Grant Support | |
ID/Acronym/Agency:
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AR35056/AR/NIAMS NIH HHS; NS28025/NS/NINDS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Diazonium Compounds; 0/Sulfanilic Acids; 2154-66-7/diazobenzenesulfonic acid; 53445-96-8/1-Carboxyglutamic Acid; 56-84-8/Aspartic Acid; 75898-26-9/beta-carboxyaspartic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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