| Isolation and characterization of rat fetal Sertoli cells. | |
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MedLine Citation:
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PMID: 2840259 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Sertoli cells were prepared from fetal rats, aged 15, 18, and 21 days. Cultures of these cells can be prepared at a purity of 85% by a method that is widely used to prepare the same cells from postnatal rats. The fetal cells are identical in appearance to postnatal Sertoli cells. Fetal Sertoli cells round up in response to (Bu)2cAMP, but not in response to FSH. Protein synthesis in the cells is not stimulated by (Bu)2cAMP or FSH. However, incorporation of [35S]methionine into secreted proteins appearing in the incubation medium is stimulated by the cyclic nucleotide, but not by FSH. Reproducible patterns for the incorporation of [35S]methionine into cellular and secreted proteins are presented as autoradiograms of one- and two-dimensional electrophoretograms. These autoradiograms show that the response of secreted proteins to (Bu)2cAMP is a general one; most or all proteins participate in the response. No clear differences were observed in the nature of the proteins synthesized when cells from fetal rats of 15, 18, and 21 days of age were compared. Fetal Sertoli cells produced approximately 2 nmol lactate/10(6) cells.h, which is approximately one fifth of the amount produced by postnatal cells. Lactate production was increased by the addition of FSH or (Bu)2cAMP to the medium. Fetal Sertoli cells also synthesize androgen-binding protein, and this activity is not increased by either FSH or (Bu)2cAMP. |
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Authors:
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H Ueda; C Ziomek; P F Hall |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Endocrinology Volume: 123 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1988 Aug |
Date Detail:
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Created Date: 1988-08-29 Completed Date: 1988-08-29 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1014-22 Citation Subset: AIM; IM |
Affiliation:
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Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Androgen-Binding Protein
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biosynthesis* Animals Bucladesine / pharmacology Cell Separation Cells, Cultured Electrophoresis, Polyacrylamide Gel Follicle Stimulating Hormone / pharmacology Gestational Age Isoelectric Point Lactates / biosynthesis Lactic Acid Male Methionine / metabolism Microscopy, Electron Molecular Weight Rats Sertoli Cells / cytology, drug effects, metabolism* Testis / cytology, embryology* |
| Grant Support | |
ID/Acronym/Agency:
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AM-28113/AM/NIADDK NIH HHS; AM-34400/AM/NIADDK NIH HHS; FO5-TWO3811/TW/FIC NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Androgen-Binding Protein; 0/Lactates; 362-74-3/Bucladesine; 50-21-5/Lactic Acid; 63-68-3/Methionine; 9002-68-0/Follicle Stimulating Hormone |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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