Document Detail


Isolation and characterization of rapidly self-renewing stem cells from cultures of human marrow stromal cells.
MedLine Citation:
PMID:  11953019     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: The adult stem cells from BM, known as non-hematopoietic mesenchymal stem cells, or marrow stromal cells (MSCs), readily generate single-cell-derived colonies, but the cultures are known to contain cells with at least two different morphologies and different properties of differentiation. Recently, we tried to identify the earliest progenitors in the cultures. METHODS: Human MSCs were plated at very low initial densities of about 3 cells/cm(2), and the growth of colonies was followed by phase microscopy. RESULTS: The two kinds of morphologically distinct cells reported by others were readily discerned: large, slowly replicating cells and spindle-shaped, more rapidly replicating cells. In addition, we observed very small cells, with diameters of only about 7 microm, that very rapidly replicated, both symmetrically and asymmetrically. The small rapidly self-renewing (RS) cells had different surface epitopes and profiles of expressed proteins than other cells in the same cultures. They also had a greater capacity for multilineage differentiation. DISCUSSION: RS cells are apparently the earliest progenitors and most rapidly replicating cells in cultures of MSCs. They have properties that appear to make them ideal candidates for studying differentiation and probably make them well-suited for cell and gene therapy.
Authors:
D J Prockop; I Sekiya; D C Colter
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cytotherapy     Volume:  3     ISSN:  1465-3249     ISO Abbreviation:  Cytotherapy     Publication Date:  2001  
Date Detail:
Created Date:  2002-04-15     Completed Date:  2003-01-24     Revised Date:  2008-04-24    
Medline Journal Info:
Nlm Unique ID:  100895309     Medline TA:  Cytotherapy     Country:  England    
Other Details:
Languages:  eng     Pagination:  393-6     Citation Subset:  IM    
Affiliation:
Center for Gene Therapy, Tulane University Health Sciences Center, 1430 Tulane Avenue, SL-99, New Orleans, LA 70112, USA.
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MeSH Terms
Descriptor/Qualifier:
Bone Marrow Cells / cytology*
Cell Culture Techniques / methods
Cell Differentiation
Cell Division
Cell Lineage
Cell Separation / methods*
Cell Size
Flow Cytometry
Humans
Mesoderm / cytology
Microscopy, Electron
Microscopy, Phase-Contrast
Stem Cells / cytology*,  ultrastructure
Tissue Therapy

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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