| Isolation and characterization of portal branch ligation-stimulated Hmga2-positive bipotent hepatic progenitor cells. | |
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MedLine Citation:
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PMID: 21075076 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hepatic stem/progenitor cells are one of several cell sources that show promise for restoration of liver mass and function. Although hepatic progenitor cells (HPCs), including oval cells, are induced by administration of certain hepatotoxins in experimental animals, such a strategy would be inappropriate in a clinical setting. Here, we investigated the possibility of isolating HPCs in a portal branch-ligated liver model without administration of any chemical agents. A non-parenchymal cell fraction was prepared from the portal branch-ligated or non-ligated lobe, and seeded onto plates coated with laminin. Most of the cells died, but a small number were able to proliferate. These proliferating cells were cloned as portal branch ligation-stimulated hepatic cells (PBLHCs) by the limiting dilution method. The PBLHCs expressed cytokeratin19, albumin, and Hmga2. The PBLHCs exhibited metabolic functions such as detoxification of ammonium ions and synthesis of urea on Matrigel-coated plates in the presence of oncostatin M. In Matrigel mixed with type I collagen, the PBLHCs became rearranged into cystic and tubular structures. Immunohistochemical staining demonstrated the presence of Hmga2-positive cells around the interlobular bile ducts in the portal branch-ligated liver lobes. In conclusion, successful isolation of bipotent hepatic progenitor cell clones, PBLHCs, from the portal branch-ligated liver lobes of mice provides the possibility of future clinical application of portal vein ligation to induce hepatic progenitor cells. |
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Authors:
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Hiroshi Sakai; Yoh-ichi Tagawa; Miho Tamai; Hiroaki Motoyama; Shinichiro Ogawa; Junpei Soeda; Takenari Nakata; Shinichi Miyagawa |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-11-12 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 403 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-20 Completed Date: 2011-01-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 298-304 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Department of Surgery, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile Ducts / growth & development Cell Proliferation Cell Separation / methods* Cells, Cultured HMGA2 Protein / analysis, biosynthesis Hepatocytes / cytology* Liver Regeneration* Mice Mice, Inbred C57BL Morphogenesis Stem Cells / chemistry, cytology* |
| Chemical | |
Reg. No./Substance:
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0/HMGA2 Protein |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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