Document Detail

Isolation and characterization of a polymerized prion protein.
MedLine Citation:
PMID:  11988079     Owner:  NLM     Status:  MEDLINE    
A polymerized form of recombinant mouse prion protein (mPrP) domain 23-231 [mPrP-(23-231)], designated mPrP-z, was generated at acidic pH (pH 2-5) in the presence of selected concentrations of denaturant (2 M guanidinium chloride or 5 M urea). This isoform of mPrP is stable in acidic solution after removal of denaturant. It can be isolated and purified using reversed-phase HPLC or size-exclusion HPLC. mPrP-z bears structural properties that partially resemble those of scrapie prion. Unlike the native mPrP-(23-231) (mPrP-N), mPrP-z exhibits a high content of beta-sheet structure, as shown by CD spectroscopy, and exists as an oligomer with an approximate molecular mass of 340000 Da, as measured by light scattering. However, similarly to mPrP-N, mPrP-z contains the intact disulphide bond and is sensitive to digestion by proteinase K.
Bao-Yuan Lu; Jui-Yoa Chang
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  364     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  2002 May 
Date Detail:
Created Date:  2002-05-03     Completed Date:  2002-06-18     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  England    
Other Details:
Languages:  eng     Pagination:  81-7     Citation Subset:  IM    
Research Center for Protein Chemistry, Institute of Molecular Medicine, University of Texas at Houston, Houston, Texas 77030, USA.
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MeSH Terms
Chromatography, High Pressure Liquid
Circular Dichroism
Endopeptidase K / metabolism
Guanidine / pharmacology
Hydrogen-Ion Concentration
Peptide Fragments / chemistry*,  isolation & purification*
Prions / chemistry*,  isolation & purification*
Protein Conformation
Protein Isoforms
Protein Structure, Secondary
Scattering, Radiation
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Time Factors
Ultraviolet Rays
Urea / pharmacology
Reg. No./Substance:
0/Disulfides; 0/Peptide Fragments; 0/Prions; 0/Protein Isoforms; 0/prion protein (23-231); 113-00-8/Guanidine; 57-13-6/Urea; EC K

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