| Isolation and characterization of a polymerized prion protein. | |
| | |
MedLine Citation:
|
PMID: 11988079 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
A polymerized form of recombinant mouse prion protein (mPrP) domain 23-231 [mPrP-(23-231)], designated mPrP-z, was generated at acidic pH (pH 2-5) in the presence of selected concentrations of denaturant (2 M guanidinium chloride or 5 M urea). This isoform of mPrP is stable in acidic solution after removal of denaturant. It can be isolated and purified using reversed-phase HPLC or size-exclusion HPLC. mPrP-z bears structural properties that partially resemble those of scrapie prion. Unlike the native mPrP-(23-231) (mPrP-N), mPrP-z exhibits a high content of beta-sheet structure, as shown by CD spectroscopy, and exists as an oligomer with an approximate molecular mass of 340000 Da, as measured by light scattering. However, similarly to mPrP-N, mPrP-z contains the intact disulphide bond and is sensitive to digestion by proteinase K. |
| | |
Authors:
|
Bao-Yuan Lu; Jui-Yoa Chang |
Related Documents
:
|
17716119 - Synthetic delivery systems for intravenous administration of nucleic acids. 6192989 - Analysis of the effect of industrial effluent on pigments, proteins, nucleic acids, and... 6541899 - Studies on simian hemorrhagic fever virus nucleocapsids. 19919149 - Single-centered hydrogen-bonded enhanced acidity (shea) acids: a new class of br?nsted ... 16333639 - Characterization of the photosynthetic apparatus in cortical bark chlorenchyma of scots... 41859 - Dialyzing room disinfection with ultra-violet irradiation. 19371139 - Isolation and characterization of leu(7)-surfactin from the endophytic bacterium bacill... 1982209 - Protection by pyroglutamic acid and some of its newly synthesized derivatives against g... 1901939 - Directed shift of fatty acids from phospholipids to triacylglycerols in hl-60 cells ind... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Biochemical journal Volume: 364 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 2002 May |
Date Detail:
|
Created Date: 2002-05-03 Completed Date: 2002-06-18 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2984726R Medline TA: Biochem J Country: England |
Other Details:
|
Languages: eng Pagination: 81-7 Citation Subset: IM |
Affiliation:
|
Research Center for Protein Chemistry, Institute of Molecular Medicine, University of Texas at Houston, Houston, Texas 77030, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Chromatography, High Pressure Liquid Circular Dichroism Disulfides Endopeptidase K / metabolism Guanidine / pharmacology Hydrogen-Ion Concentration Light Mice Peptide Fragments / chemistry*, isolation & purification* Prions / chemistry*, isolation & purification* Protein Conformation Protein Isoforms Protein Structure, Secondary Scattering, Radiation Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Time Factors Ultraviolet Rays Urea / pharmacology |
| Chemical | |
Reg. No./Substance:
|
0/Disulfides; 0/Peptide Fragments; 0/Prions; 0/Protein Isoforms; 0/prion protein (23-231); 113-00-8/Guanidine; 57-13-6/Urea; EC 3.4.21.64/Endopeptidase K |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: A novel pathway for adrenergic stimulation of cAMP-response-element-binding protein (CREB) phosphory...
Next Document: Identification of an initiator-like element essential for the expression of the tissue inhibitor of ...