| Isolation and characterization of a novel cytokinesis-deficient mutant in Dictyostelium discoideum. | |
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MedLine Citation:
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PMID: 8844408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cytokinesis is a dramatic event in the life of any cell during which numerous mechanisms must coordinate the legitimate and complete mechanical separation into two daughter cells. We have used Dictyostelium discoideum as a model system to study this highly orchestrated event through genetic analysis. Transformants were generated using a method of insertional mutagenesis known as restriction enzyme-mediated integration (REMI) and subsequently screened for defects in cytokinesis. Mutants isolated in a similar screen suffered a disruption in the myosin II heavy chain gene, a protein known to be essential for cytokinesis and in a novel gene encoding a rho-like protein termed racE [Larochelle et al., 1996]. In the screen reported here we isolated a third type of mutant, called 10BH2, which also had a complete defect in cytokinesis. 10BH2 mutant cells are able to propagate on tissue culture plates by fragmenting into smaller cells by a process known as traction-mediated cytofission. However, when grown in suspension culture, 10BH2 cells fail to divide and become large and multinucleate. Phenotypic characterization of the mutant cells showed that other cytoskeletal functions are preserved. The distribution of myosin and actin is identical to wild type cells. The cells can chemotax, phagocytose, cap crosslinked receptors, and contract normally. However, the 10BH2 mutants are unable to complete the Dictyostelium developmental program beyond the finger stage. The mutant cells contain functional genes for myosin II heavy and light chains and the racE gene. Thus, based on these findings, we conclude that 10BH2 represents a novel cytokinesis-deficient mutant. |
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Authors:
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K K Vithalani; J D Shoffner; A De Lozanne |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of cellular biochemistry Volume: 62 ISSN: 0730-2312 ISO Abbreviation: J. Cell. Biochem. Publication Date: 1996 Aug |
Date Detail:
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Created Date: 1996-12-18 Completed Date: 1996-12-18 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8205768 Medline TA: J Cell Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 290-301 Citation Subset: IM |
Affiliation:
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Department of Cell Biology, Duke University Medical Center, Durham, North Carolina 27710, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/U46204; U46205 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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analysis Amino Acid Sequence Animals Base Sequence Cell Division / genetics* Cloning, Molecular Concanavalin A DNA, Protozoan / analysis, genetics* Dictyostelium / cytology*, genetics, growth & development GTP-Binding Proteins / analysis Gene Dosage Gene Rearrangement Genes, Protozoan / genetics Molecular Sequence Data Mutagenesis, Insertional Mutation* Myosins / analysis, genetics RNA, Transfer / genetics Repetitive Sequences, Nucleic Acid / genetics Sequence Analysis, DNA rac GTP-Binding Proteins* |
| Grant Support | |
ID/Acronym/Agency:
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GM 48745/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Actins; 0/DNA, Protozoan; 0/RacE protein, Dictyostelium discoideum; 11028-71-0/Concanavalin A; 9014-25-9/RNA, Transfer; EC 3.6.1.-/GTP-Binding Proteins; EC 3.6.4.1/Myosins; EC 3.6.5.2/rac GTP-Binding Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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