| Isolation and characterization of a novel H1.2 complex that acts as a repressor of p53-mediated transcription. | |
| | |
MedLine Citation:
|
PMID: 18258596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Linker histone H1 has been generally viewed as a global repressor of transcription by preventing the access of transcription factors to sites in chromatin. However, recent studies suggest that H1 can interact with other regulatory factors for its action as a negative modulator of specific genes. To investigate these aspects, we established a human cell line expressing H1.2, one of the H1 subtypes, for the purification of H1-interacting proteins. Our results showed that H1.2 can stably associate with sets of cofactors and ribosomal proteins that can significantly repress p53-dependent, p300-mediated chromatin transcription. This repressive action of H1.2 complex involves direct interaction of H1.2 with p53, which in turn blocks p300-mediated acetylation of chromatin. YB1 and PURalpha, two factors present in the H1.2 complex, together with H1.2 can closely recapitulate the repressive action of the entire H1.2 complex in transcription. Chromatin immunoprecipitation and RNA interference analyses further confirmed that the recruitment of YB1, PURalpha, and H1.2 to the p53 target gene Bax is required for repression of p53-induced transcription. Therefore, these results reveal a previously unrecognized function of H1 as a transcriptional repressor as well as the underlying mechanism involving specific sets of factors in this repression process. |
| | |
Authors:
|
Kyunghwan Kim; Jongkyu Choi; Kyu Heo; Hyunjung Kim; David Levens; Kimitoshi Kohno; Edward M Johnson; Hugh W Brock; Woojin An |
Related Documents
:
|
14515206 - Corepressor smrt specifically represses the transcriptional activity of orphan nuclear ... 12070146 - The adenovirus e1a protein targets the saga but not the ada transcriptional regulatory ... 22784096 - Mcm7 expression predicts post-operative prognosis for hepatocellular carcinoma. 12869186 - Gene regulation by tetracyclines. constraints of resistance regulation in bacteria shap... 17644806 - High expression of human telomerase reverse transcriptase in high-grade intraepithelial... 21839716 - Egfr expression stratifies oligodendroglioma behavior. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-07 |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 283 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2008 Apr |
Date Detail:
|
Created Date: 2008-03-31 Completed Date: 2008-05-22 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
|
Languages: eng Pagination: 9113-26 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry and Molecular Biology, University of Southern California Keck School of Medicine, Los Angeles, CA 90089, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetylation Chromatin / metabolism* DNA-Binding Proteins / metabolism E1A-Associated p300 Protein / metabolism Gene Expression Regulation / physiology, radiation effects* Hela Cells Histones / metabolism* Humans Nuclear Proteins / metabolism RNA Interference Repressor Proteins / metabolism* Transcription Factors / metabolism Transcription, Genetic / physiology* Tumor Suppressor Protein p53 / metabolism* bcl-2-Associated X Protein / biosynthesis |
| Chemical | |
Reg. No./Substance:
|
0/BAX protein, human; 0/Chromatin; 0/DNA-Binding Proteins; 0/Histones; 0/Nuclear Proteins; 0/PURA protein, human; 0/Repressor Proteins; 0/TP53 protein, human; 0/Transcription Factors; 0/Tumor Suppressor Protein p53; 0/YBX1 protein, human; 0/bcl-2-Associated X Protein; EC 2.3.1.48/E1A-Associated p300 Protein; EC 2.3.1.48/EP300 protein, human |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: CO2 fixation kinetics of Halothiobacillus neapolitanus mutant carboxysomes lacking carbonic anhydras...
Next Document: Neph1, a component of the kidney slit diaphragm, is tyrosine-phosphorylated by the Src family tyrosi...