| Isolation and characterization of a new fucoganglioside accumulated in precancerous rat liver and in rat hepatoma induced by N-2-acetylaminofluorene. | |
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MedLine Citation:
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PMID: 7085643 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A novel fucoganglioside has been found to be accumulated in the liver of rats fed N-2-acetylaminofluorene before development of hepatoma. This new fucoganglioside persisted in hepatoma in vivo but was completely absent in normal rat liver as well as in livers of rats fed the nonhepatic carcinogen, acetylaminophenanthrene, and a tumor promoter, trichloro-2,2-bis-(chlorophenyl)ethane. The ganglioside was isolated by high performance liquid chromatography and the structure was determined by methylation analysis, sequential degradation by various exoglycosidases, and by direct probe mass spectrometry of the permethylated derivative. This ganglioside, present in precancerous liver and in hepatoma in vivo, was identified as having a new structure with a substitution identical with blood group B determinant as shown below: (formula, see text) A second fucoganglioside was detected in lower quantity in precancerous liver and in hepatoma in vivo but not in control tissue. This ganglioside co-migrated with the fucoganglioside isolated from H-35 hepatoma cells in vitro whose structure was identified (Baumann, H., Nudelman, E., Watanabe, K., and Hakomori, S. (1979) Cancer Res. 39, 2637-2643) as fucosylated GM1 ganglioside (Fuc alpha 1 leads to 2Gal beta 1 leads to 4GalNAc beta 1 leads to 4 [NeuAc alpha 2 leads to 3]Gal beta 1 leads to 4Glc beta 1 leads to 1Cer). The results indicate that synthesis of new fucolipids is already induced at an early stage during the process of chemical carcinogenesis in rat liver which could be a unique membrane marker for diagnosis and therapy of a hepatoma and its premalignancy. |
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Authors:
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E H Holmes; S Hakomori |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 257 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1982 Jul |
Date Detail:
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Created Date: 1982-08-26 Completed Date: 1982-08-26 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 7698-703 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Acetylaminofluorene
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pharmacology* Animals Gangliosides / biosynthesis, isolation & purification* Glycolipids / isolation & purification Liver / drug effects, metabolism* Liver Neoplasms, Experimental / chemically induced, metabolism* Male Mass Spectrometry Precancerous Conditions / metabolism* Rats Rats, Inbred F344 Reference Values |
| Grant Support | |
ID/Acronym/Agency:
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F32 CA06580/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Gangliosides; 0/Glycolipids; 0/fucogangliosides; 53-96-3/2-Acetylaminofluorene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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