Document Detail


Isolation and characterization of a new compound from Prunus mume fruit that inhibits cancer cells.
MedLine Citation:
PMID:  16536585     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
An active compound that inhibits cancer cells was isolated from the fruit of Prunus mume, and its structure and in vitro activities were characterized. The n-hexane fraction obtained from methanol extracts exhibited the strongest inhibitory effect on the growth of cancer cells. From the n-hexane fraction, a new compound named B-1 was purified through preparative thin-layer chromatography, ODS column chromatography, and reverse phase high-performance liquid chromatography and its structure was analyzed by fast atom bombardment mass spectrometry and 1H and 13C NMR. The molecular formula of B-1 was C19H22O6 {2-hydroxy-1-[(7-hydroxy-2-oxo-2H-chromen-6-yl)methyl]-2-methylpropyl-(2Z)-3-methyl-but-e-enoate:prunate}, and the IC50 value was in the range of 39-58 microg/mL in descending order of the cancer cell lines Hep-2, SW-156, HEC-1-B, and SK-OV-3. B-1 exhibited 81-96% inhibition at a concentration level of 100 microg/mL against all cells, based on an 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay. However, B-1 showed little effect against normal cells with only 23% or less growth inhibition at 100 microg/mL. Thus, B-1 has a highly specific inhibitory effect against cancer cells but little effect against normal cells. When the cancer cell lines Hep-2 and SK-OV-3 were incubated with B-1 for 72 h, most of the tested cells suffered strong growth inhibition. The compound has the potential to be developed as a nutraceutical.
Authors:
Jong Tae Jeong; Jae-Hak Moon; Keun-Hyung Park; Chul Soo Shin
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of agricultural and food chemistry     Volume:  54     ISSN:  0021-8561     ISO Abbreviation:  J. Agric. Food Chem.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-03-15     Completed Date:  2006-04-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0374755     Medline TA:  J Agric Food Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2123-8     Citation Subset:  IM    
Affiliation:
Bohae R&D Center, Bohae Brewery Co. Ltd., Yongin 449-171, South Korea.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / isolation & purification,  pharmacology
Benzopyrans / isolation & purification*,  pharmacology
Butyric Acids / isolation & purification*,  pharmacology
Cell Division / drug effects*
Cell Line, Tumor
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fruit / chemistry*
Humans
Kidney Neoplasms
Laryngeal Neoplasms
Magnetic Resonance Spectroscopy
Mice
NIH 3T3 Cells
Neoplasms / pathology*
Prunus / chemistry*
Spectrometry, Mass, Fast Atom Bombardment
Chemical
Reg. No./Substance:
0/2-hydroxy-1-((7-hydroxy-2-oxo-2H-chromen-6-yl)methyl)-2-methylpropyl-(2Z)-3-methyl-but-e-enoate; 0/Antineoplastic Agents; 0/Benzopyrans; 0/Butyric Acids

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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