Document Detail

Isolation and characterization of neural stem cells from the neonatal rat cochlear nucleus.
MedLine Citation:
PMID:  21258945     Owner:  NLM     Status:  Publisher    
Neural stem cells have been identified in multiple parts of the postnatal mammalian brain, as well as in the inner ear. No investigation of potential neural stem cells in the cochlear nucleus has yet been performed. The aim of this study was to investigate potential neural stem cells from the cochlear nucleus by neurosphere assay and in histological sections to prove their capacity for self-renewal and for differentiation into progenitor cells and cells of the neuronal lineage. For this purpose, cells of the cochlear nucleus of postnatal day 6 rats were isolated and cultured for generation of primary neurospheres. Spheres were dissociated and cells analyzed for capacity for mitosis and differentiation. Cell division was detected by cell-counting assay and BrdU incorporation. Differentiated neural progenitor cells showed distinct labeling for Nestin and for Atoh1. Positive staining of ß-III Tubulin, glial fibrillary acid protein (GFAP) and myelin basic protein (MBP) showed differentiation into neurons, astrocytes and oligodendrocytes. Furthermore, Nestin- and BrdU-labeled cells could also be detected in histological sections. In conclusion, the isolated cells from the cochlear nucleus presented all the features of neural stem cells: cell division, presence of progenitor cells and differentiation into different cells of the neuronal lineage. The existence of neural stem cells may add to the understanding of developmental features in the cochlear nucleus.
Kristen Rak; Natalia V Wasielewski; Andreas Radeloff; Johannes Völkers; Agmal Scherzed; Sibylle Jablonka; Rudolf Hagen; Robert Mlynski
Related Documents :
1725695 - Identification of pyruvate carboxylase in 3t3-l1 cells by transblot and its correlation...
3043175 - Isolation and characterization of a variant myoblast cell line that is temperature sens...
1946315 - Ultrastructural differentiation of sodium butyrate-treated human pancreatic adenocarcin...
15078875 - The relative contribution exerted by af-1 and af-2 transactivation functions in estroge...
2987005 - Dna topoisomerase i and ii activities during cell proliferation and the cell cycle in c...
20559625 - Involvement of hsp70, a stress protein, in the resistance of long-term culture of pc12 ...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-1-22
Journal Detail:
Title:  Cell and tissue research     Volume:  -     ISSN:  1432-0878     ISO Abbreviation:  -     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-1-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of Oto-Rhino-Laryngology, Plastic, Aesthetic and Reconstructive Head and Neck Surgery and the Comprehensive Hearing Center, University of Wuerzburg, Josef-Schneider-Straße 11, 97080, Wuerzburg, Germany.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Dicyemid's dilemma: structure versus genes. The unorthodox structure of dicyemid reproduction.
Next Document:  A mono-institutional prospective study on the effectiveness of a specialist psychotherapeutic interv...