| Isolation and characterization of a near-haploid human cell line. | |
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MedLine Citation:
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PMID: 10527618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Mammalian somatic cells are usually diploid. Occasional rare human tumors have been shown to have a hypodiploid karyotype. We have isolated a near-haploid subclone (P1-55) from a heterogeneous human leukemia cell line, KBM-7. These near-haploid cells have approximately half the human diploid DNA content and have a haploid karyotype except for a disomy of chromosome 8 (25, XY, +8, Ph(+)). This cell line maintains a majority of cells with a near-haploid karyotype for at least 12 weeks in culture. By serial subcloning, we have isolated near-haploid subclones that maintain ploidy for at least 8 months in culture. Near-haploid cells can also be efficiently isolated from mixed ploidy cultures by size selection. The availability of this human near-haploid cell line should facilitate the genetic analysis of cultured human cells. |
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Authors:
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M Kotecki; P S Reddy; B H Cochran |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 252 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 1999 Nov |
Date Detail:
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Created Date: 1999-12-07 Completed Date: 1999-12-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 273-80 Citation Subset: IM |
Copyright Information:
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Copyright 1999 Academic Press. |
Affiliation:
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Department of Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Haploidy* Humans Karyotyping Leukemia / genetics*, pathology* Tumor Cells, Cultured* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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