Document Detail


Isolation and characterization of a near-haploid human cell line.
MedLine Citation:
PMID:  10527618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Mammalian somatic cells are usually diploid. Occasional rare human tumors have been shown to have a hypodiploid karyotype. We have isolated a near-haploid subclone (P1-55) from a heterogeneous human leukemia cell line, KBM-7. These near-haploid cells have approximately half the human diploid DNA content and have a haploid karyotype except for a disomy of chromosome 8 (25, XY, +8, Ph(+)). This cell line maintains a majority of cells with a near-haploid karyotype for at least 12 weeks in culture. By serial subcloning, we have isolated near-haploid subclones that maintain ploidy for at least 8 months in culture. Near-haploid cells can also be efficiently isolated from mixed ploidy cultures by size selection. The availability of this human near-haploid cell line should facilitate the genetic analysis of cultured human cells.
Authors:
M Kotecki; P S Reddy; B H Cochran
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental cell research     Volume:  252     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  1999 Nov 
Date Detail:
Created Date:  1999-12-07     Completed Date:  1999-12-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  273-80     Citation Subset:  IM    
Copyright Information:
Copyright 1999 Academic Press.
Affiliation:
Department of Physiology, Tufts University School of Medicine, 136 Harrison Avenue, Boston, Massachusetts, 02111, USA.
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MeSH Terms
Descriptor/Qualifier:
Haploidy*
Humans
Karyotyping
Leukemia / genetics*,  pathology*
Tumor Cells, Cultured*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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