| Isolation and characterization of myosin from cloned rat glioma and mouse neuroblastoma cells. | |
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MedLine Citation:
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PMID: 132225 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Myosin has been isolated from the clonal lines of murine neuroblastoma and rat glioma cells. Partial characterization of the two cellular myosins indicates that both possess the following properties: (1) the same elution position as rabbit skeletal muscle myosin by Sepharose 4B chromatography; (2) the presence of heavy (molecular weight about 200,000) and light subunit polypeptides by sodium dodecyl sulfate polyacrylamide gel electrophoresis; (3) EDTA and Ca2+ activated but Mg2+-inhibited ATPase activity in 0.6 M KCl; and (4) binding to rabbit skeletal muscle F-actin which is inhibited by Mg2+-ATP. For both mouse neuroblastoma and rat glioma cells, approximately 0.5-1.5% of the total cell protein is present as myosin. Cellular myosin appears to be indistinguishable in quantity and biochemical properties regardless of whether it is isolated from monolayer or suspension neuroblastoma cells. |
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Authors:
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C Miller; W M Kuehl |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 108 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1976 May |
Date Detail:
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Created Date: 1976-08-23 Completed Date: 1976-08-23 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 115-24 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Actins
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isolation & purification Adenosine Triphosphatases Animals Calcium Catalysis Chemical Phenomena Chemistry Clone Cells Edetic Acid Electrophoresis, Polyacrylamide Gel Glioma / analysis* Magnesium Mice Muscles / analysis Myosins / isolation & purification* Neuroblastoma / analysis* Rabbits Rats Sodium Dodecyl Sulfate |
| Chemical | |
Reg. No./Substance:
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0/Actins; 151-21-3/Sodium Dodecyl Sulfate; 60-00-4/Edetic Acid; 7439-95-4/Magnesium; 7440-70-2/Calcium; EC 3.6.1.-/Adenosine Triphosphatases; EC 3.6.4.1/Myosins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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