Document Detail


Isolation and characterization of mutants of cowpea Rhizobium defective in nitrate uptake system.
MedLine Citation:
PMID:  6194663     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this communication, two mutants of cowpea Rhizobium CB756 (NR-18 and NR-23), defective in nitrate uptake system, were described. They failed to grow in a medium where nitrate was the sole source of nitrogen and unable to show nitrate reductase activity in cell suspensions. However, they showed nitrate reductase activity (to the level of wild type) in cell-free extract preparations. Thus it appears that in these mutants, the transport genes of nitrate uptake were affected and the synthesis of nitrate induced permease, as its role in nitrate uptake is indicated here, is defective.
Authors:
R K Singh; R M Singh
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Acta microbiologica Polonica     Volume:  32     ISSN:  0137-1320     ISO Abbreviation:  Acta Microbiol. Pol.     Publication Date:  1983  
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  7610362     Medline TA:  Acta Microbiol Pol     Country:  POLAND    
Other Details:
Languages:  eng     Pagination:  31-5     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Genes, Bacterial*
Membrane Transport Proteins / genetics
Mutation
Nitrate Reductases / metabolism
Nitrates / metabolism*
Rhizobium / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Membrane Transport Proteins; 0/Nitrates; EC 1.7.-/Nitrate Reductases

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