Document Detail


Isolation and characterization of a monoclonal antibody directed against type 1 fimbriae organelles from Escherichia coli.
MedLine Citation:
PMID:  6194117     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have isolated a mouse immunoglobulin M (IgM) monoclonal antibody directed against type 1 fimbriae from the Escherichia coli K-12-derived strain CSH50. Antibody specificity was demonstrated by (i) the ability of fimbriate but not nonfimbriate bacteria to compete with solid-phase purified fimbriae for antibody binding in an enzyme-linked immunosorbent assay, (ii) the visualized binding of antibody to fimbriae alone by electron microscopy, and (iii) the appearance in a radioimmunoprecipitation assay of a single electrophoretic band comigrating with pure type 1 fimbriae. The monoclonal antibody was further characterized by immunoblot analysis and compared with previously prepared rabbit anti-fimbrial antisera. Whereas the monospecific but polyvalent antisera recognized both fimbrial monomeric subunits and non-disaggregated fimbriae organelles, the monoclonal antibody recognized only the intact organelles even when the samples were prepared under nondenaturing conditions. The monoclonal antibody, therefore, might be directed against an epitope spanning two (or more) adjacent fimbrial subunits.
Authors:
B I Eisenstein; J R Clements; D C Dodd
Related Documents :
22907367 - Affinity determination of biotinylated antibodies by flow cytometry.
18766577 - Protective effects of hn and f glycoprotein-specific monoclonal antibodies on experimen...
8110797 - Differentiation of intrahepatic membrane-bound and secretory apolipoprotein b by monocl...
22137827 - Missing: a diagnostic technique to enumerate antigen-specific t cells.
22381917 - The clinical significance of autoantibodies to the proliferating cell nuclear antigen (...
9524937 - Production of a monoclonal antibody reacted broadly with feline calicivirus field isola...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Infection and immunity     Volume:  42     ISSN:  0019-9567     ISO Abbreviation:  Infect. Immun.     Publication Date:  1983 Oct 
Date Detail:
Created Date:  1983-11-23     Completed Date:  1983-11-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0246127     Medline TA:  Infect Immun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  333-40     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Bacterial / immunology,  isolation & purification*
Antibodies, Monoclonal / immunology,  isolation & purification*
Antibody Specificity
Bacterial Proteins / immunology
Enzyme-Linked Immunosorbent Assay
Epitopes / immunology
Escherichia coli / immunology,  ultrastructure*
Fimbriae, Bacterial / immunology*
Immunoglobulin M / immunology
Mice
Microscopy, Electron
Grant Support
ID/Acronym/Agency:
AI-19670/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal; 0/Bacterial Proteins; 0/Epitopes; 0/Immunoglobulin M
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Common lipopolysaccharide specificity: new type of antigen residing in the inner core region of S- a...
Next Document:  Antigenic relatedness of two strains of hepatitis A virus determined by cross-neutralization.