Document Detail

Isolation and characterization of monoacetyldiglycerides from bovine udder.
MedLine Citation:
PMID:  10588942     Owner:  NLM     Status:  MEDLINE    
Monoacetyldiglycerides (MADGs) were isolated from an animal tissue, bovine udder, by solvent extraction and silica gel column chromatography. Monoacetyldiglyceride structures were identified using a variety of 1-D and 2-D NMR techniques and collision-induced dissociation (CID) tandem mass spectrometry coupled with fast atom bombardment. CID of sodium-adducted molecular ions ([M+ Na](+)) generated numerous types of product ions providing information on the double bond position of fatty acyl groups as well as fatty acid compositions. Structural analysis led to the classification of the MADGs isolated from bovine udder as 1-palmitoyl-2-lauroyl-3-acetyl-rac-glycerol, 1, 2-dimyristoyl-3-acetyl-rac-glycerol, 1-palmitoyl-2-myristoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-myristoyl-3-acetyl-rac-glycerol, 1-palmitoyl-2-palmitoleoyl-3-acetyl-rac-glycerol, 1, 2-dipalmitoyl-3-acetyl-rac-glycerol, 1-linoleoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-palmitoleoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-stearoyl-2-palmitoyl-3-acetyl-rac-glycerol, 1-oleoyl-2-linoleoyl-3-acetyl-rac-glycerol, 1, 2-dioleoyl-3-acetyl-rac-glycerol, and 1-stearoyl-2-oleoyl-3-acetyl-rac-glycerol.
J K Limb; Y H Kim; S Y Han; G J Jhon
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of lipid research     Volume:  40     ISSN:  0022-2275     ISO Abbreviation:  J. Lipid Res.     Publication Date:  1999 Dec 
Date Detail:
Created Date:  2000-02-10     Completed Date:  2000-02-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0376606     Medline TA:  J Lipid Res     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  2169-76     Citation Subset:  IM    
Department of Chemistry, Division of Molecular Life Science and Center for Cell Signaling Research, Ewha Womans University, Seoul 120-750, Korea.
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MeSH Terms
Chromatography, Gel
Diglycerides / chemistry*,  isolation & purification*
Mammary Glands, Animal / chemistry*
Nuclear Magnetic Resonance, Biomolecular
Spectrometry, Mass, Fast Atom Bombardment
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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