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Isolation and characterization of mitochondria from goat hearts.
MedLine Citation:
PMID:  23105498     Owner:  NLM     Status:  PubMed-not-MEDLINE    
Abstract/OtherAbstract:
Cardiac mitochondria provide energy for the contraction/relaxation cycle. The aim of our study was to isolate and characterize mitochondria from Caprine hearts under control and in-vitro induced ischemia. A decrease in activities of all the enzymes was observed in the ischemic models. Further characterization of proteins was done by SDS-PAGE and BN-PAGE. Lipids have been characterized by analyzing the phospholipids by HPTLC and fatty acids by GLC in both groups. Our results indicated that injury occurs early in the course of ischemia and progresses during ischemia. TBARS and carbonyl content have also been measured. The in-vitro effects of fatty acids have been studied on the enzymes and complexes of mitochondria.
Authors:
Tester F Ashavaid; Neena S Kumbhat
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Indian journal of clinical biochemistry : IJCB     Volume:  20     ISSN:  0970-1915     ISO Abbreviation:  Indian J Clin Biochem     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2012-10-29     Completed Date:  2012-10-30     Revised Date:  2013-05-30    
Medline Journal Info:
Nlm Unique ID:  8708303     Medline TA:  Indian J Clin Biochem     Country:  India    
Other Details:
Languages:  eng     Pagination:  72-9     Citation Subset:  -    
Affiliation:
Research Laboratories, P.D. Hinduja National Hospital and Medical Research Centre, Veer Savarkar Marg, 400 016 Mahim, Mumbai India.
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