Document Detail

Isolation and characterization of a major allergen from oriental mustard seeds, BrajI.
MedLine Citation:
PMID:  1804799     Owner:  NLM     Status:  MEDLINE    
A 2 S albumin from oriental mustard (Brassica juncea) seeds has been isolated and characterized as an allergen. This protein, BrajI, was recognized by human IgE from mustard-sensitive individuals, as proved by using two different enzyme immunoassays. BrajI was found to be closely related to Sin a I, the major allergen from yellow mustard seeds. Many fractions with molecular weights ranging from 16,000 to 16,400 and with differences in charge were separated by ion-exchange chromatography. They exhibited small but significant amino acid composition differences for Glx, Val, Ile, Lys, and Arg contents. The heterogeneity of BrajI can be explained by size and charge differences of its heavy and light chains. All of the isoallergenic forms of BrajI gave a single precipitation band in double diffusion immunoassays when using a Sin a-I-specific rabbit polyclonal serum.
M A González de la Peña; L Menéndez-Arias; R I Monsalve; R Rodríguez
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International archives of allergy and applied immunology     Volume:  96     ISSN:  0020-5915     ISO Abbreviation:  Int. Arch. Allergy Appl. Immunol.     Publication Date:  1991  
Date Detail:
Created Date:  1992-05-01     Completed Date:  1992-05-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0404561     Medline TA:  Int Arch Allergy Appl Immunol     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  263-70     Citation Subset:  IM    
Departamento de Bioquímica y Biología Molecular I, Facultad de Ciencias Químicas, Universidad Complutense, Madrid, Spain.
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MeSH Terms
2S Albumins, Plant
Albumins / chemistry*,  immunology
Allergens / chemistry*,  immunology
Amino Acids / analysis
Antibodies, Monoclonal / immunology
Antigens, Plant
Brassica / immunology*
Hypersensitivity / immunology
Immunoenzyme Techniques
Immunoglobulin E / metabolism
Molecular Weight
Plant Proteins / chemistry,  immunology*
Reg. No./Substance:
0/2S Albumins, Plant; 0/Albumins; 0/Allergens; 0/Amino Acids; 0/Antibodies, Monoclonal; 0/Antigens, Plant; 0/Bra j protein, Brassica juncea; 0/Plant Proteins; 37341-29-0/Immunoglobulin E

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