Document Detail


Isolation and characterization of the major acidic glycosphingolipids from the liver of the English sole (Parophrys vetulus). Presence of a novel ganglioside with a Forssman antigen determinant.
MedLine Citation:
PMID:  3257757     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Acidic glycosphingolipids of the liver of English sole, Parophrys vetulus, have been isolated and characterized by 1H nuclear magnetic resonance spectroscopy, methylation analysis, and by direct probe electron-impact and fast atom bombardment mass spectrometry. In addition to the acidic glycosphingolipids with known structures (sulfatide, GM4, GM3, GM2, and GD1a), two fractions of a major monosialosylganglioside with TLC mobility slower than GM1 were isolated and characterized as having the following structure. (Formula:q see text). The structure represents a novel combination of a terminal Forssman disaccharide (GalNAc alpha 1----3GalNAc beta 1----3R) and a GM1 ganglioside core linked together. The identity of the terminal Forssman disaccharide was further established by TLC immunostaining with an anti-Forssman monoclonal antibody. This antibody showed strongly positive staining of the ganglioside only after removal of the sialic acid. Thus, the II3NeuAc residue inhibited antibody binding to the terminal disaccharide unit. Analysis of the ceramide moieties of both fractions indicated a predominance of 16:0, 22:1, 22:0, and 24:1 fatty acids in the faster migrating form and 16:0, 18:0, and 18:1 in the slower form in combination with d18:1 sphingosine.
Authors:
G K Ostrander; S B Levery; S Hakomori; E H Holmes
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  263     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  1988 Mar 
Date Detail:
Created Date:  1988-04-06     Completed Date:  1988-04-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3103-10     Citation Subset:  IM    
Affiliation:
Pacific Northwest Research Foundation, Seattle, Washington 98104.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens, Heterophile / analysis*
Ceramides / analysis
Chemical Fractionation
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Fatty Acids / analysis
Flatfishes / metabolism*
Forssman Antigen / analysis*
G(M1) Ganglioside / analysis*,  immunology
Gangliosides / analysis
Glycosphingolipids / analysis*,  immunology
Immunoassay
Liver / analysis*
Magnetic Resonance Spectroscopy
Mass Spectrometry
Sulfoglycosphingolipids / analysis
Grant Support
ID/Acronym/Agency:
CA42505/CA/NCI NIH HHS; ES02190/ES/NIEHS NIH HHS; ES04370/ES/NIEHS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, Heterophile; 0/Ceramides; 0/Fatty Acids; 0/Gangliosides; 0/Glycosphingolipids; 0/Sulfoglycosphingolipids; 37758-47-7/G(M1) Ganglioside; 9013-60-9/Forssman Antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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