Document Detail


Isolation and characterization of isopimaric acid-degrading bacteria from a sequencing batch reactor.
MedLine Citation:
PMID:  8795202     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We isolated two aerobic, gram-negative bacteria which grew on the diterpene resin acid isopimaric acid (IpA) as the sole carbon source and electron donor. The source of the isolates was a sequencing batch reactor treating a high-strength process stream from a paper mill. The isolates, IpA-1 and IpA-2, also grew on pimaric and dehydroabietic acids, and IpA-1 grew on abietic acid. Both strains used fatty acids, but neither strain used camphor, sitosterol, or betulin. Strain IpA-1 grew anaerobically with nitrate as an electron acceptor. Strains IpA-1 and IpA-2 had growth yields of 0.19 and 0.23 g of protein per g of IpA, respectively. During growth, both strains transformed IpA carbon to approximately equal amounts of biomass, carbon dioxide, and dissolved organic carbon. In both strains, growth on IpA induced an enzymatic system which caused cell suspensions to transform all four of the above resin acids. Cell suspensions of IpA-1 and IpA-2 removed IpA at rates of 0.56 and 0.13 mumol mg of protein-1 h-1, respectively. Cultures and cell suspensions of both strains failed to completely consume pimaric acid and yielded small amounts of an apparent metabolite from this acid. Cultures and cell suspensions of both strains yielded large amounts of three apparent metabolites from dehydroabietic acid. Analysis of 16S rDNA sequences indicated that the isolates are distinct members of the genus Pseudomonas sensu stricto.
Authors:
A E Wilson; E R Moore; W W Mohn
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Applied and environmental microbiology     Volume:  62     ISSN:  0099-2240     ISO Abbreviation:  Appl. Environ. Microbiol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  1996-10-17     Completed Date:  1996-10-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7605801     Medline TA:  Appl Environ Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3146-51     Citation Subset:  IM    
Affiliation:
Department of Microbiology and Immunology, University of British Columbia, Vancouver, Canada.
Data Bank Information
Bank Name/Acc. No.:
GENBANK/X96787;  X96788
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MeSH Terms
Descriptor/Qualifier:
Base Sequence
Diterpenes / metabolism*
Gram-Negative Aerobic Bacteria / growth & development,  isolation & purification*,  metabolism
Molecular Sequence Data
Phylogeny
Suspensions
Chemical
Reg. No./Substance:
0/Diterpenes; 0/Suspensions
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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